Measurement of. Telecommunications traffic billed hereunder shall be (i) in actual conversation time as specified in FCC terminating FGD Switched access tariffs for Local Traffic and (ii) in accordance with applicable tariffs for all other types of Telecommunications traffic.
Measurement of. A number of previous studies report the use of SDS-PAGE gels to quantitate the amount of [*] present in tissue samples, and to measure breakdown of [*] (eg. Fry et al., Acta Physiol. Scand. 1997; 161:473). The specificity of the fragments observed using SDSPAGE are confirmed by Western blot. We will utilise this technique to investigate the levels of intact and degraded [*] in a number of skeletal muscle tissues obtained from lean, obese and diabetic Psammomys obesus. Frozen muscle samples will be serially sectioned using 40~Lm sections. 5 sections from each sample will be placed into 1.0ml of lysing buffer containing 10% (w/v) glycerol, 5% (v/v) b-mercaptoethanol and 2.3% (w/v) SDS in 62.5 nmo1/L Tris-HCL (pH 6.8), and heated for 10 min at 65°C. 15m1 of the extract will be loaded on 3-6°io gradient SDS-polyacrylamide gels (pH 8.4) with 3% stacking gels (pH 6.8) and electrophoresed for 21h at 120V. Protein bands will be visualised using silver staining and quantitated using optical densitometry. Western blots will be used to verify the identity of [*] bands on the gels, using a monoclonal anti-[*] clone T11 antibody (available from Sigma, St. Louis, MO). Immunoreactivity will be visualised with an anti-mouse secondary antibody and horseradish peroxidase chloro development reagent 4-chloro-l-napthol (Bio-Rad, Hercules, CA). [*] content will also be measured in C2C12 and/or L6 cells
Measurement of terminating Local minutes begins when the terminating Local entry Switch receives answer supervision from the called End User Customer's End Office Switch indicating the called End User Customer has answered. The measurement of terminating call usage over Local trunks ends when the terminating Local entry switch receives disconnect supervision from either the called End User Customer's End Office Switch, indicating the called End User Customer has disconnected, or NT&T’s Point of Interconnection, whichever is recognized first by the entry switch. This is commonly referred to as "conversation time." The Parties will only charge for actual minutes of use and/or fractions thereof of completed calls. Minutes of use are aggregated at the end of the billing cycle by End Office Switch and rounded to the nearest whole minute.
Measurement of. Payment: Unless specified above, the Contractor will be paid for all its services required above for the “Annual” and “As-Required” drills on a per sessions basis and will include all Work, including providing necessary equipment, personnel and paperwork to satisfy all Laws and directives. Upon the beginning of each year in the Contract, the Contractor will submit a schedule of payment to compensate it under this section. Such yearly payment schedule will be approved by NYCDOT before payment can be made.
