Southern Blotting Sample Clauses

Southern Blotting. Genomic DNA was extracted from tails, and 15 g of DNA was digested with the appropriate enzyme in a 30 l reaction at 37C overnight. Digested genomic DNA was loaded on a 1% agarose gel in 1x TAE buffer, and run overnight at 40 volts. The next day, the gel was stained with ethidium bromide for 20 minutes and then a picture was taken with a ruler. Proper digestion was detected by a smear of DNA representing all size fragments. The gel was washed in 500 ml of 0.25 HCL for 15 minutes, and then washed in 500 ml of 0.5N NaOH/1M NaCl for 15 minutes. The gel was then neutralized by washing three times in 0.5M Tris (pH 8.0) with 3M NaCl for 30 minutes each. DNA was transferred to a nitrocellulose membrane in 20X SSC (3M NaCl/0.3M sodium citrate) overnight at RT. The membrane was cross linked by UV light, and pre-hybridized in 10- 15 ml of hyb buffer (GE healthcare, NIF939) at 65C for 1 hour. A probe was radioactivity-labeled by using the Rediprime II kit (GE healthcare, RPN1636), and once the labeled probe was spun down, 100 l of H2O, 500 l of salmon sperm, and 25 l of COT DNA was added. The probe was boiled for 10 minutes, and added to the bottle containing the membrane and hybridized at 65C overnight. The membrane was washed in 0.1xSSC/0.1% SDS twice at RT and subsequently twice at 65C for 15 minutes per wash. The membrane was placed on film (Kodak BioMax MsFilm 8294985) at -80C overnight and then developed on the Konica MinoLTA developer (SRX-101A).
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  • Within Southern DHB External to Southern DHB Nurse Manager • Relevant associated groups, local and regional • Service Manager • Healthcare providers • Unit team managers • Healthcare patients/consumer • Clinical Charge Nurse • National Speciality groups • Clinical Leaders • NZNO, PSA and other relevant professional colleges of nursing and midwifery • All Nursing and Midwifery Staff • Nursing Council/Midwifery council • Allied Health Staff • Nurse Educators • Medical Staff • Clinical Support Staff • Infection Prevention and Control • Occupational Health & Safety PERSON SPECIFICATION The expertise required for a person to be fully competent in the role. Position specific competencies: ESSENTIAL DESIRABLE Education and Qualifications (or equivalent level of learning) • Be registered with the Nursing Council of New Zealand as a registered Nurse • Hold a current Nursing Council of New Zealand annual practising certificate • Working towards Post graduate qualifications at a minimum of a Diploma or/and a masters degree • Advanced learning and contemporary knowledge in area of specialist practice. • Experience • Experience and credibility with the health care team as a professional resource in speciality/practice area • Networking effectively in area of specialty/ practice. • Demonstrated ability to articulate evidence based nursing advice to a Multidisciplinary Team. • Minimum of 3 years practice within the defined scope of clinical speciality/practice Knowledge and Skills • Exercise independent nursing judgement, apply advanced assessment skills, initiate appropriate clinical interventions, evaluate outcomes, advise others and provide sound rationale for actions in area of speciality/practice. • Work independently and in the interdisciplinary team, using evidence based evidence and international best practice, in area of speciality. • Initiate health promotion activities to encourage productive health behaviours and xxxxxx the strengths and potential of patients/consumer and staff. • Be a professional leader and role model, with proven ability to inspire, motivate and develop others, in area of speciality. • Be champions for, and promote the profession of nursing. • Current issues for nursing and health in NZ and international context. • Current government policies, codes, guidelines, and legislation impacting on area of speciality. • Current nursing professional standards and codes. • Southern DHB current vision, goals and objectives. • Principles of the Treaty of Waitangi and biculturalism. Personal Qualities A reasonable level of fitness is required to cope with the demanding physical requirements of the job. The following denote the key physical requirements for the position: standing, walking, bending, sitting, stairs, simple grasping, fine manipulation, operating machinery / equipment, lifting, overhead reaching, carrying, pushing / pulling, twisting, climbing / balancing, crouching / squatting, manual handling of people, other reaching, and ability to participate in personal restraint if required. KEY RESULT AREAS: Key Accountabilities: Example of successful delivery of duties and responsibilities Leadership Works collaboratively to achieve the organisation, nursing and specialty vision and values, and promotes quality patient outcomes. Inspires, motivates and encourages nurses to initiate and maintain best practice in their specialty area. Takes the leadership role through both direct and indirect patients/consumer care delivery. Provides senior nursing leadership for the specialty service and consultation to a broad range of hospital, community and professional groups to achieve positive outcomes for patient or population group. Supports a culture of evaluation and the ongoing quality improvement of nursing practice. Is actively involved in professional activities such as research, scholarship and policy development at both a local and national level. Staff are encouraged and supported in their work as it relates to the area of speciality practice. Xxxxxxx and participates in peer review processes, case review and reflective practice. Team members are motivated and stimulated. Evaluates current practice, identifies deficits/needs and introduces quality measures. Participates actively in team meetings. Leads system change to improve health outcomes through evidence based practice. Participates in clinical governance activities Works closely with team members from a wide range of clinical disciplines, helping to create a productive responsive patient focused team that is able to deliver cost effective quality care. Represents the specialty nursing perspective. Involvement with workforce development and succession planning for the service from a nursing perspective. Employs and models transformational leadership style and coaches nurses to develop own leadership skills. Clinical Practice Promotes and role models expert nursing care either directly or indirectly, especially for those with complex needs. Provides expert nursing knowledge in the management of patients/consumer care within a defined area of speciality practice, working with the patient, family/whanau or other health professionals to provide timely nursing care to optimise outcomes. Exercises independent nursing judgement, applies advanced assessment skills, initiates appropriate clinical interventions, evaluates outcomes, advises others and provides sound rationale for actions in area of speciality Prescribes advanced evidenced based nursing therapeutics, pharmacological/ non- pharmacological interventions, diagnostic measures, equipment, procedures, and treatments to meet the needs of patients/consumer, families and groups, in accordance with professional preparation, institutional policies and scope of practice. Acts as a mentor, guides and supports others in the speciality in their assessment, clinical decision making, implementation, evaluation and documentation of care. Facilitates/initiates inter service collaboration and promotes integrated care between secondary and primary services to improve patient care. Undertakes extended interventions as authorised by NCNZ Networks nationally and internationally to identify and implement nursing advances and changes in speciality practice. Maintains a significant presence in the clinical area as a resource, role model, mentor and advocate. Facilitates / initiates inter-service collaboration to improve patient care Provides input into the development of care co-ordination initiatives Professional portfolio maintained PDRP at level 4. Meets Nursing Council NZ requirements for APC Clinical Practice Works with nursing staff to continuously improve nursing practice and patient/consumer outcomes. Role models expert evidenced based clinical practice, is seen as highly effective, progressive and knowledgeable. Provides leadership, role models and guides others in nursing practice that is evidenced based and consistent with the principles of the Treaty of Waitangi. Gains support for changes to nursing practice that are based on: • quality improvement activity reports • patient/consumer feedback • contractual/ legislative changes • treatment changes current evidence based best practice. Participates in auditing nursing quality standards, policies, procedures and guidelines and developing action plans relevant to speciality. Clinical Practice Articulates the ethical, cultural and statutory requirements of practice and initiates / responds to changes from the internal and external environment. Provides input into, and helps interpret requirements of new legislation /guidelines. Supports clinicians in identifying ethical dilemmas working through a decision making framework. Attends and provides input into and feedback from relevant committees. Nursing specialty submissions are made in the development of relevant organisational and national policies. Actively manages risk. Assess the quality of nursing practice in the clinical setting. Collaborates on any changes required. To demonstrate effective interpersonal relationship skills [Description where applicable] Establishes, maintains and concludes therapeutic interpersonal relationships with patient/consumer. Practices nursing in a negotiated partnership with the patient/consumer and family/whanau where and when possible. Communicates effectively with patients/consumer and family/whanau and members of the health care team. Maintains privacy and confidentiality in accordance with HIP Code, DHB policies and procedures etc. Communicates in an appropriate and professional manner, verbal and written. Privacy Act, Informed Consent and Code of Rights adhered to. Abides by NCNZ Code of Conduct and Professional Boundaries guidelines. To participate in inter-professional health care Leads nursing and interdisciplinary groups in designing and implementing innovative, cost effective patient care and evidence-based change Provides a primary point of contact within the speciality for patients/consumer and health professionals Initiates referrals to other members of the health care team in a timely manner. Evaluates results of interventions using recommended criteria, revises management/treatment and initiates timely referral/care with relevant services/agencies Is a competent consultant for interdisciplinary client base Contributes to research and the dissemination of findings where possible Contributes to the development of interdisciplinary standards of practice and evidence-based guidelines Demonstrate leadership in establishing collaborative relationships within and across departments, hospitals, primary and secondary health to promote optimal patient/consumer health and safety and continuity of care. Initiates timely referrals to other services in a timely and thorough manner. Engages in team and MDT meetings as appropriates. Initiate appropriate audit processes. Consistently participates/leads multi-disciplinary team meetings and family conferences, representing the nursing perspective of patient/consumer needs, and enacting outcomes appropriately. Leads in activities which monitor/audit delivery of quality patient care e.g. Certification processes, and current or retrospective nursing audits. Engages in submission processes. Education Assists in providing for the educational needs of staff within the specialised area of practice. Collaborates in the development of education programmes related to the area of speciality knowledge and skill. Develops professional networks Locally, Nationally and Internationally to maintain current knowledge of trends and developments in specialty area. Participates in the educational plan for the speciality. Participates in nursing forums where relevant to area of practice Colleagues are informed of relevant nursing professional trends and issues. Participates in collaborative educational networks to meet professional and service needs. Shares specialist knowledge and skills in formal and informal education activities and ensures that nurses are supported in their development of culturally safe practice. Assists in evaluating educational programmes offered, that focus on the area of speciality practice, to ensure content is evidence based and reflective of current thinking. Nurses are kept informed of relevant evidence based practice and issues, including ethical issues. Education Provides educational support for healthcare providers and patients. Disseminates specialty knowledge at both a Local and National/International level to ensure healthcare providers and consumers are informed about the service CNSs provide, hence facilitating access to the speciality support they require. Shares specialist knowledge and skills in formal and informal education activities and ensures that nurses are supported in their development of culturally safe practice. Acts as a resource person for patients/consumer and health professionals. Collaborates with Nurse Educators and line managers to identify staff training needs and to develop an educational plan to address these needs. Assists in the orientation and preceptor ship of new nurses and student nurses. Provides clinical guidance and mentoring to nursing and allied health colleagues. Teaching sessions are provided for health care providers e.g. general practitioners, practice nurses, staff in rural areas etc, as relevant to area of speciality. Education programmes are developed for patients/consumers to assist them to achieve their best clinical outcomes. This may include health promotion / wellness activities. Assists in the compilation of resource material for educational purposes for patients/consumer, families and healthcare professionals updating as required, to promote the sharing of current evidence-based information. Research Promotes quality nursing care through research-based practice Relevant nursing and related research is read, critiqued and discussed as a means of supporting the development of quality nursing practice. Participates in conferences relevant to area of speciality, as agreed line manager. Patients/consumer research which challenges practice at local and national forums. Information is communicated to staff within Southern DHB, to maximise the benefit for the patient/consumer and organisation. Research Role models the application of evidence based best practice principles in own practice, and assist others in research related activities. Demonstrates ability to interpret and implement research findings relevant to area of speciality practice. Provides research based clinical management options for complex clinical situations / issues. There is evidence of staff participation in research related activities where appropriate and authorised.

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  • Dewatering 4.7.1 Where a part of a site is affected by surface water following a period of rain, thus rendering some areas unsafe for productive work, consistent with the Employer’s obligations under the OH&S Act, all non- trades employees shall assist in ‘dewatering’ their own work site or area if it is so affected. Such work to be paid at single time rates. Productive work will continue in areas not so affected.

  • Modern Slavery You hereby affirm your compliance with the Modern Slavery Xxx 0000 and associated guidance. You confirm (a) that you have read, are familiar with and shall not perform an act or omission which is in contravention with, the letter or spirit of the Act; and (b) you carry out regular, meaningful and comprehensive due diligence procedures and have internal policies in place to address any suspected human rights abuse in your business and Group where applicable.

  • Washtenaw Community College Eastern Michigan University Xxxxxx Xxxxxxxxxx College of Engineering & Technology Student Services BE 214 xxx_xxxxxxxx@xxxxx.xxx; 734.487.8659 734.973.3398

  • Cheating Cheating is prohibited, and will result in immediate action and termination without compensation.

  • Community Mental Health Center Services Assertive Community Treatment Staffing Full Time Equivalents Community Mental Health Center March 2021 December 2020 Nurse Masters Level Clinician/or Functional Support Worker Peer Specialist Total (Excluding Psychiatry) Psychiatrist/Nurse Practitioner Total (Excluding Psychiatry) Psychiatrist/Nurse Practitioner 01 Northern Human Services - Wolfeboro 1.00 0.00 0.00 0.57 6.81 0.27 8.27 0.25 01 Northern Human Services - Berlin 0.34 0.31 0.00 0.00 3.94 0.14 4.17 0.14 01 Northern Human Services - Littleton 0.00 0.14 0.00 0.00 3.28 0.29 3.31 0.29 02 West Central Behavioral Health 0.60 1.00 0.00 0.00 5.40 0.30 5.90 0.30 03 Lakes Region Mental Health Center 1.00 1.00 0.00 1.00 5.00 0.40 7.00 0.38 04 Riverbend Community Mental Health Center 0.50 1.00 6.90 1.00 10.40 0.50 10.50 0.50 05 Monadnock Family Services 1.91 2.53 0.00 1.12 11.17 0.66 10.32 0.62 06 Greater Nashua Mental Health 1 1.00 1.00 3.00 1.00 7.65 0.15 8.50 0.15 06 Greater Nashua Mental Health 2 1.00 1.00 4.00 1.00 8.65 0.15 8.50 0.15 07 Mental Health Center of Greater Manchester-CTT 1.33 10.64 2.00 0.00 19.95 1.17 21.61 1.21 07 Mental Health Center of Greater Manchester-MCST 1.33 9.31 3.33 1.33 19.95 1.17 25.27 1.21 08 Seacoast Mental Health Center 1.00 1.10 5.00 1.00 10.10 0.60 10.10 0.60 09 Community Partners 0.50 0.00 3.40 0.88 7.28 0.70 7.41 0.70 10 Center for Life Management 1.00 0.00 2.28 1.00 6.71 0.46 6.57 0.46 Total 12.51 29.03 29.91 9.33 126.29 6.96 137.43 6.96 2b. Community Mental Health Center Services: Assertive Community Treatment Staffing Competencies Community Mental Health Center Substance Use Disorder Treatment Housing Assistance Supported Employment March 2021 December 2020 March 2021 December 2020 March 2021 December 2020 01 Northern Human Services - Wolfeboro 1.27 1.27 5.81 6.30 0.00 0.40 01 Northern Human Services - Berlin 0.74 0.74 3.29 3.29 0.00 0.23 01 Northern Human Services - Littleton 1.43 1.29 2.14 2.14 1.00 1.00 02 West Central Behavioral Health 0.20 0.20 4.00 0.40 0.60 0.60 03 Lakes Region Mental Health Center 1.00 3.00 5.00 7.00 2.00 2.00 04 Riverbend Community Mental Health Center 0.50 0.50 9.40 9.50 0.50 0.50 05 Monadnock Family Services 1.69 1.62 4.56 4.48 0.95 1.18 06 Greater Nashua Mental Health 1 6.15 7.15 5.50 6.50 1.50 1.50 06 Greater Nashua Mental Health 2 5.15 5.15 6.50 6.50 0.50 0.50 07 Mental Health Center of Greater Manchester-CCT 14.47 15.84 13.96 15.62 2.66 2.66 07 Mental Health Center of Greater Manchester-MCST 6.49 7.86 15.29 19.28 1.33 2.66 08 Seacoast Mental Health Center 2.00 2.00 5.00 5.00 1.00 1.00 09 Community Partners 1.20 1.20 4.50 4.50 1.00 1.00 10 Center for Life Management 2.14 2.14 5.42 5.28 0.29 0.29 Total 44.43 49.96 90.37 99.39 13.33 15.52 Revisions to Prior Period: None. Data Source: Bureau of Mental Health CMHC ACT Staffing Census Based on CMHC self-report. Notes: Data compiled 04/26/2021. For 2b: the Staff Competency values reflect the sum of FTEs trained to provide each service type. These numbers are not a reflection of the services delivered, but rather the quantity of staff available to provide each service. If staff are trained to provide multiple service types, their entire FTE value is credited to each service type.

  • Bedding The manufacturing, repairing, covering, re-covering of mattress bases, mattresses, spring mattresses, overlays, bolsters, pillows, cushions for studio couches, spring units, box-spring mattresses and studio couches, but excluding the manufacturing of bedding made mainly of metal and/or plastic materials.

  • Cloud storage DSHS Confidential Information requires protections equal to or greater than those specified elsewhere within this exhibit. Cloud storage of Data is problematic as neither DSHS nor the Contractor has control of the environment in which the Data is stored. For this reason:

  • Drainage Systems (1) Clear culvert inlets, outlets, and sediment catching basins.

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