Luciferase activity Sample Clauses

Luciferase activity. After 48 h incubation, the medium was removed and the cells were lysed by adding 100 µl of Glo lysis buffer,1X, to each well for 5 min. The cells lysate (5 µl) was assayed for protein content using the Bio-Rad DC protein assay kit I. The rest of the cell lysate was transferred to a white 96-well plate and the luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (according to the manufacturer’s protocol) and the Microtiter plate luminometer (Dynex technologies,