P asma AVP Sample Clauses

P asma AVP. As described before (Xx Xxxxxx et al. \oo3), within 7 days of the CPRS interview, blood samples were drawn on a single day under standardised conditions between o9.oo a.m. and 9.3o a.m. and between 3.3o p.m. and 4.oo p.m. All patients refrained from ingesting alcohol and from undertaking strenuous physical exercise (sports) for 1\ h before the study. They sat down 15 min before venipuncture. Smoking was not allowed for 3o min before venipuncture; eating and drinking were allowed ad libitum. Blood was collected in 1o-mL vacutainer tubes and immediately stored at 4 °C. Within 3o min, plasma was separated in a cooled centrifuge and stored at -8o °C. The determination of plasma AVP was based on radioimmunoassay (RIA) following peptide extraction using C-8 Bond ElutR cartridges (Analytichem International, Harbor City, CA, USA RIA was performed using a rabbit antiserum (coded W1E) with the following cross- reactivities: vasotocin 1oo%; (Cyt6)AVP-(3–9) 5o%; (pGlu4, Cyt6)AVP-(4–9) \5%; (Cyt6)AVP- (5–9) 13%; AVP-(1–8), AVP-(1–7) and oxytocin undetectable. The detection limit of the extracted assay was o.5 pg/mL plasma, and the intra-and inter-assay coefficients of variation were 9.9% and 15.9%, respectively. Patient and control samples were coded and assayed in a single run. For each patient, mean daytime plasma AVP level (plasma AVP) was computed from the morning and afternoon values. Above-normal plasma AVP was defined as any value ›5.56 pg/mL corresponding with the ROC analysis relating familial depression to above-normal plasma AVP (Goekoop et al. \oo6).