Performance of the test. Carry out all hydrolysis experiments by employ- ing one of the procedures described in this paragraph. Prepare the test solu- tions as described in paragraph (b)(2)(i) of this section at pH 3.00, 7.00, and 11.00 ±0.05, and determine the initial test chemical concentration (Co) in trip- licate. Analyze each reaction mixture in triplicate at regular intervals, em- ploying one of the following proce- dures:
Performance of the test. Both cells to be employed should be rinsed with the blank solution and then filled with same. The instrument should be set to scan at a rate appropriate for the re- quired wavelength resolution and the spectrum of the blank recorded. The sample cell should then be rinsed and filled with the test solution and the scanning repeated, preferably on the same spectrum chart, to display the baseline. The test should be carried out at 25 °C.
