Plasmid pDNA amplification and purification Sample Clauses

Plasmid pDNA amplification and purification. The pGL3 control vector encoding for luciferase gene was amplified using an electro-competent E.coli JM109 strain cultured on Ampicillin LB (Xxxxx-Xxxxxxx) agar for 8 h and then subcultured in LB broth for 12-16 h. The bacterial cells were harvested and the pDNA yield was then extracted and purified using a Qiagen Endofree Plasmid Mega Kit according to the manufacturer’s instructions (appendix 1). The resulting DNA was stored in 1X TE buffer (10 mM Tris (pH 8), 1 mM EDTA)
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