Present address. District of…………...…………….……..….....…...., Municipality of , p.c. , City/ town ………...............……………....….., Str. .........................................................№ ............, bl. ............, entr. , floor ........, ap , telephone:....................................., e-mail , hereinafter referred to as THE INSURED, on the other hand, this contract was made and entered into, as follows:
Present address. District of…………...…………….……..….....…...., Municipality of , p.c………, City/ town ………...............………..., Str......................................................№ ............, bl. ............, entr. , floor ........, ap………….., telephone:....................................., e-mail ,
Present address. Department of Surgery, UCSD Xxxxxxx Xxxxxx, Xxx Xxxxx, XX 00000. METHODS MATERIALS. Male Xxxxxxx-Xxxxxx rats weighing 200-300g were obtained from Xxxxxxx River (Raleigh, NC). The procurement and method of use of the animals in this study were approved by the Duke University Medical Center Review Board for the welfare of animals. Gas tanks with standard 95% O2/5% CO2 were obtained from National Welders Supply Co., Inc. (Charlotte, NC). Collagenase (Type P) was obtained from Boehringer Mannheim Co. (Indianapolis, IN). Highly purified bovine serum albumin (BSA, fraction V) free of fatty adds and insulin-like activity and a11 chemicals for buffer and encapsulation solutions were purchased from Sigma Chemical Co. (St. Louis, MO). Crystalline pork insulin was obtained courtesy of Dr. Xxxxxx Chance, Xxx Lilly (Indianapolis, IN). Monoiodinated 125I-insulin was obtained from New England Nuclear (Boston, MA). ISLET ISOLATION. Islets were isolated by the collagenase digestion procedure described by Xxxx ET AL. [6]. Rats were anesthetized with intraperitoneal pentobarbital. Laparotomy was performed, and the pancreatic duct cannulated. The pancreas was then distended by infusion of 15 ml Hank's balanced salt solution (HBSS). Pancreatectomy was performed, and the distended pancreas was digested in collagenase solution (1.5 mg/ml) at 37(0)C for 25 min. The digested tissue was filtered through a mesh and then washed several times [TABLE]
1. Glucose-stimulated insulin secretion in control islets (n = 4). Following a 1-h preperifusion of unencapsulated islets with KRB containing 3.3 mM (basal) glucose, basal effluent perifusate was collected over 20 min. The glucose concentration in the perifusate was then raised to 16.7 mM and after 30 min of perifusion with sample collection, the glucose concentration was reduced to basal during another 20 min of perifusion. in chilled HBSS and placed under a dissecting microscope. At least 400 islets/experiment were purified by being handpicked and placed in chilled HBSS until imeroencapsulation.
Present address. Occupation: .............................................................................................................................................................................
Present address. Occupation: ............................................................................................................................................................................. Executed this day of 200
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