Procedure Overview Sample Clauses
Procedure Overview. The normative procedure followed by implementations claiming support for this annex is described in Section B.3, Extensions to Q.933 Annex A. This overview provides an informative description of the procedures. If there is a discrepancy between this text and the text in section B.3, the text in section xxx shall apply. The additional configuration information is transmitted by the DCE when: • a PVC is created, • a PVC configuration is changed, and • following recovery from a service affecting condition. The additional information is transmitted to the DTE in a Full Status message sent by the DCE in response to a received Status Enquiry message. This information is encoded in two additional information elements: The Link Layer Core Parameters (LLCP) IE and the Priority and Service Class Parameters (PSCP) IE. The LLCP IE and the PSCP IE follow the PVC Status IE. A description of the LLCP IE and the PSCP IE is provided in [X.36]. To guarantee delivery of the additional configuration information, the additional information elements are transmitted in every Full Status message until the delivery of the additional information is confirmed through comparison of Send and Receive sequence numbers. The DCE will stop transmitting the additional information elements only when a DTE-originated Status Enquiry message is received that contains a Receive sequence number that equals a Send sequence number that tracks the previously transmitted Full Status message containing the additional information elements. See Figure 2 for an illustration of an acknowledge delivery. (Report Type: Full Status) Send Sequence #: 84 Receive Sequence #: 28 DLCI 16: PVC, LLCP, and PSCP IE Send Sequence #: 29 Receive Sequence #: 84 (Report Type: Link integrity verification only) Send Sequence #: 85 Receive Sequence #: 29
Procedure Overview. The procedure starts with the Lysis/Binding step, where a specifically formulated chaotropic salt-based Lysis/Binding buffer is used to simultaneously lyse cells, degrade protein components, and bind the gDNA to the surface of the paramagnetic beads. A three-step washing procedure, including two washing buffers, is performed for the removal of proteins, detergents, and other impurities. During the last step, gDNA is released from the magnetic beads using the Elution buffer.
