Seed destruction Sample Clauses
Seed destruction. The maintenance of a transgenic rice seed log is the responsibility of ICS. The inventory of transgenic seeds will be reviewed annually. Seed stocks designated by Ceres as no longer needed will be destroyed by autoclaving as described in SOP R7. Their destruction will be recorded in the seed log.
1. SOP R1: Agrobacterium-mediated Rice Transformation
Seed destruction. The maintenance of a transgenic [***] seed log is the responsibility of ICS. The inventory of transgenic seeds will be reviewed annually. Seed stocks designated by Ceres as no longer needed will be destroyed by autoclaving as described in SOP R7. Their destruction will be recorded in the seed log. Protocol Number: Leave this blank. Property of Ceres, Inc. Protocol Name: [***] Transformation Authors: ▇▇▇▇▇▇▇ ▇▇▇▇▇ Date Issued: 12/30/[***] Approved By: Chuan-▇▇▇ ▇▇ Version SOP [***]: [***] Transformation
1. Purpose:
2. References:
3. Safety:
3.1. Follow universal lab safety technique
3.2. Proper disposal of transgenic waste in designated receptacles
3.2.1. Dispose the unused Agrobacterium culture in the biological waste container
3.2.2. Dispose all the excess callus and plant materials in the biological waste container
Seed destruction. The maintenance of a transgenic [***] seed log is the responsibility of ICS. The inventory of transgenic seeds will be reviewed annually. Seed stocks designated by Ceres as no longer needed will be destroyed by autoclaving as described in SOP R7. Their destruction will be recorded in the seed log. Protocol Number: Leave this blank. Property of Ceres, Inc. Protocol Name: [***] Transformation Authors: ▇▇▇▇▇▇▇ ▇▇▇▇▇ Date Issued: 12/30/[***] Approved By: Chuan-▇▇▇ ▇▇ Version SOP [***]: [***] Transformation
1. Purpose:
2. References:
3. Safety:
3.1. Follow universal lab safety technique
3.2. Proper disposal of transgenic waste in designated receptacles
3.2.1. Dispose the unused Agrobacterium culture in the biological waste container
3.2.2. Dispose all the excess [***] and plant materials in the biological waste container
4. [***] Integrity Concerns
4.1. Misidentification • To avoid misidentification, request HTPC group to use printed labels on tubes when handing over Agrobacterium to transformation group. The label should contain Gemini ID, date of inoculation and the initials of the person who inoculated the culture.
4.2. Mislabeling: To avoid mislabeling • Agrobacterium culture tubes during re-inoculation, keep the tubes that belong to the same Gemini ID together. Check the label on the tubes before each inoculation. • Always check the label on the lid of the Petri dish before subculturing. • Sub-culture just one construct at a time to avoid mix up. Once you finish subculturing a construct, label the plates and then go on to the next construct. • Print labels to avoid mislabeling the Petri dish.
4.3. Errors in tracking • Print labels to avoid mislabeling. • Count the number of plates used for sub-culturing each construct. In the next stage, make sure that the count matches. For example, if you used 15 plates to subculture one construct from co-cultivation to selection, make sure that there are 15 plates when you transfer the [***] from selection to regeneration. • Enter all the data in the work sheet, so it is easy to refer. Protocol Number: Leave this blank. Property of Ceres, Inc. Protocol Name: [***] Transformation Authors: ▇▇▇▇▇▇▇ ▇▇▇▇▇ Date Issued: 12/30/[***] Approved By: Chuan-▇▇▇ ▇▇ Version
4.4. Errors in disposition • Check the database to verify if you are handing over the transformants for the right person who requested the transformation of that particular construct. • Ask the requestor, to sign off those transformants to indicate that they have r...