Special stains. Based on the re- sults of the general staining, selected sites and cellular components shall be further evaluated by use of specific techniques. If H&E screening does not provide such information, a battery of stains shall be used to assess the fol- lowing components in all appropriate required samples: Neuronal body (e.g., Xxxxxxxx’x gallocyanin), axon (e.g., Xxxxxx’x Luxol Fast Blue), and neurofibrils (e.g., Bielchosky). In addi- tion, nerve fiber teasing shall be used. A section of normal tissue shall be in- cluded in each staining to assure that adequate staining has occurred. Any changes shall be noted and representa- tive photographs shall be taken. If xx- xxxxx are observed, the special tech- niques shall be repeated in the next lower treatment group until no further lesions are detectable.
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