AMENDMENT DATED JUNE 1, 2008 TO INVESTMENT SUB-ADVISORY AGREEMENT

Exhibit D(16)

AMENDMENT

DATED JUNE 1, 2008 TO

INVESTMENT SUB-ADVISORY AGREEMENT

For MML NASDAQ-100^® Fund

WHEREAS, Massachusetts Mutual Life Insurance Company (“MassMutual”) and Northern Trust Investments, Inc. (now known as Northern Trust Investments, N.A.) (the “Sub-Adviser”) entered into an Investment Sub-Advisory Agreement (the “Agreement”), effective as of January 31, 2003 relating to the MML OTC 100 Fund (now known as the MML NASDAQ-100^® Fund) (the “Fund”); and

WHEREAS, Section 20 of the Agreement permits the Agreement to be amended by a written instrument approved in writing by both parties;

NOW THEREFORE, IT IS AGREED THAT:

1. Capitalized terms used herein but not otherwise defined shall have the meanings given to those terms in the Agreement.

2. The Sub-Adviser will not consult with any other sub-adviser to a fund for which MassMutual serves as investment adviser concerning transactions for the Fund in securities or other assets.

3. Except as expressly amended hereby, all provisions of the Agreement remain in full force and effect and are unchanged in all other respects.

4. This Amendment may be executed in one or more counterparts, each of which shall be deemed to be an original and, all of which, when taken together, shall constitute one and the same instrument.

IN WITNESS WHEREOF, the parties hereto intending to be legally bound have caused this Amendment to be executed by their duly authorized officers or other representatives as of the day and year first above written.

MASSACHUSETTS MUTUAL LIFE   INSURANCE COMPANY				NORTHERN TRUST INVESTMENTS, N.A.
By:		/s/ Xxxx Xxxxxxx		By:		/s/ Xxxxx X. Xxxxxxxxx
		Name: Xxxx Xxxxxxx				Name:  Xxxxx X. Xxxxxxxxx
		Title: Corporate Vice President				Title:    Senior Vice President

Accepted and Agreed to by:

MML SERIES INVESTMENT FUND

on behalf of MML NASDAQ-100^® Fund

By:		/s/ Xxxxxxxx Xxxxxxxxx
		Name:   Xxxxxxxx Xxxxxxxxx
		Title:  CFO and Treasurer