END USER LICENCE AGREEMENTEnd User License Agreement • December 16th, 2014
Contract Type FiledDecember 16th, 2014Reads are sampled from the mutated reference T′ in a non-random way. Sam- pling begins at one end of the reference and reads are sampled from the reference at a regular interval. Every second read is then replaced with its reverse com- plement, in order to simulate reads being created from both the forward and the reverse strand of the reference. The number of reads generated depends on the sampling interval specified. Coverage of the reference is determined by the length of the reads and the sampling interval. Any reads found to contain unknown nucleotides (’N’) are discarded (Algorithm 2).