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License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of LeidenJanuary 9th, 2009
Figure 3. A) Growth curves of yeast cells expressing wild-type human adenosine A2B receptors. Each curve represents the growth of yeast cells as a response to [NECA] in an individual well of a 96-well microtiter plate (liquid medium assay). Based on the yeast growth in various wells with different concentrations of NECA, a dose-response curve B) can be obtained, in this case at 35 h of growth. C) Yeast growth assay (solid medium assay) to detect constitutive and agonist-induced receptor activation. Growth of yeast cells expressing the wild-type and the F84S mutant adenosine A2B receptor is shown. The agonist NECA is required to induce growth of yeast cells expressing the wild-type receptor. The F84S mutant receptor is constitutively active as indicated by the fact that yeast growth occurs in the absence of NECA. Upon addition of NECA the growth of yeast cells expressing this mutant receptor is further enhanced.
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of LeidenJanuary 9th, 2009
Figure 3. A) Growth curves of yeast cells expressing wild-type human adenosine A2B receptors. Each curve represents the growth of yeast cells as a response to [NECA] in an individual well of a 96-well microtiter plate (liquid medium assay). Based on the yeast growth in various wells with different concentrations of NECA, a dose-response curve B) can be obtained, in this case at 35 h of growth. C) Yeast growth assay (solid medium assay) to detect constitutive and agonist-induced receptor activation. Growth of yeast cells expressing the wild-type and the F84S mutant adenosine A2B receptor is shown. The agonist NECA is required to induce growth of yeast cells expressing the wild-type receptor. The F84S mutant receptor is constitutively active as indicated by the fact that yeast growth occurs in the absence of NECA. Upon addition of NECA the growth of yeast cells expressing this mutant receptor is further enhanced.
