Common Contracts
1 similar null contracts
ASSET: Analysing and Striking the Sensitivities of Embryonal Tumours ASSET WP 3 – microRNA expression in ETs: rRegulation of by ET oncogenes identification of targets & effects Deliverable D 3.1 - miRNAs regulating ET Cell viability Due date of...February 14th, 2013
MicroRNAs (miRNAs) are central connections between the genome, transcriptome and proteome. Their crucial roles in cancer are increasingly recognised. The objective of this deliverable, and task 3.2. was ‘global approaches to identify critical miRNAs for embryoid tumor (ET) cell viability using high-throughput screening (HTS)’. VTT Medical Biotechnology is equipped to conduct high throughput screening in a 384 well plate format using a robotic HTS screening system (Figure 1). The objective was to make high-throughput cell-based miRNA transfection screens with a comprehensive library of >800 pre-mirs (Dharmacom miRIDIAN Mimics (N=810) As VTT has also a library of miRNA inhibitors (Dharmacom miRIDIAN inhibitors (N=896)) these were also screened. These screens will generate data concerning ET cell proliferation.
