Transfection Medium definition

Transfection Medium means the solutions of nutrients used in mammalian cell culture, as more fully set out in Exhibit 3.
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Examples of Transfection Medium in a sentence

  • If the plated cells were maintained in serum-containing medium, wash the monolayers twice with TC100 without serum and then add 1 ml of TC100 without serum (or ES Transfection Medium) to each 35 mm dish or well of a 12-well plate.

  • After 24 hours, the cells were at confluency, or 0.5 x 106 cells per plate, and we washed the cells with shRNA Transfection Medium.

  • For each co-transfection in either a 35mm dish or well of a 12-well plate, you need to mix in a polystyrene tube, in the following order: 100 µl serum free medium (TC100 preferably or serum-free growth medium or ES Transfection Medium) 100 ng virus DNA from the kit (flashBAC™ or BacPAK6) [5µl]500 ng transfer plasmid (5 µl lacZ transfer vector from flashBAC™ kit) or YOUR transfer vector1.2 µl baculoFECTIN II Mix (total volume = 111.2 µl) and leave at room temperature for 15 mins.

  • Notwithstanding anything to the contrary, it is understood and agreed that, on an annual basis, Licensee shall pay to Lonza the fee set forth in Section 6.3 of the Lonza License for Licensee’s research, development and commercialization of Products utilizing or incorporating only Lonza’s GS System and Transfection Medium System and the Research License Fee set forth in Section 6.2.2 herein for Licensee’s research, development and commercialization of Products utilizing or incorporating the Technology.

  • If the plated cells were maintained in serum-containing medium, wash the monolayers twice with TC100 without serum and then add 1mL of TC100 without serum (or ES Transfection Medium) to each 35mm dish/6-well plate or well of a 12-well plate.

  • After incubation period, add appropriate amount of siRNA Transfection Medium to the tube containing the siRNA-siRNA Transfection Reagent Complex and mix gently.

  • If Transfection Medium = Growth Medium, Indicate Growth medium in Description and N/A PN and/or LN column.

  • Stability of the Size Particles in Transfection Medium The influence of transfection medium complexes on size was studied by incubating the particles in transfection culture medium.

  • The Lonza License shall remain valid, absent termination by either Lonza or Licensee, in accordance with its terms, and shall govern Licensee’s research, development and commercialization of Products utilizing or incorporating only Lonza’s GS System and Transfection Medium System, as those terms are defined in the Lonza License.

  • RAW264.7 cells (5 x 105/mL) were cultured in six-well plates for 6 hr and the transfected with IRG1 siRNA (100 nM) or control siRNA (100 nM) using siRNA Transfection Medium (Santa Cruz) following the instructions of the manufacturer.

Related to Transfection Medium

  • Prescription medication means any medication that requires a physician prescription before it may be obtained from a pharmacist.

  • Membrane filtration means a pressure or vacuum driven separation process in which particulate matter larger than 1 micrometer is rejected by an engineered barrier, primarily through a size-exclusion mechanism, and which has a measurable removal efficiency of a target organism that can be verified through the application of a direct integrity test. This definition includes the common membrane technologies of microfiltration, ultrafiltration, nanofiltration, and reverse osmosis.

  • Nonprescription medication means a drug or medication authorized pursuant to federal or state law for general distribution and use without a prescription in the treatment of human disease, ailments, or injuries.

  • Polystyrene foam means blown polystyrene and expanded and

  • Electrostatic application means the application to a substrate of charged atomized paint droplets that are deposited by electrostatic attraction.

  • Exfiltration means any unauthorized release of data from within an information system. This includes copying the data through covert network channels or the copying of data to unauthorized media.

  • Polystyrene foam adhesive means an aerosol adhesive designed to bond polystyrene foam to substrates.

  • Sedimentation means a process for removal of solids before filtration by gravity or separation.

  • Backflow prevention device means a safety device used to prevent pollution or contamination of the water supply due to the reverse flow of water from the irrigation system.

  • Disinfection profile means a summary of Giardia lamblia inactivation through the treatment plant.

  • Conventional filtration treatment means a series of processes including coagulation, flocculation, sedimentation, and filtration resulting in substantial particulate removal.

  • Automated data acquisition and handling system or "DAHS" means that component of the continuous emission monitoring system, or other emissions monitoring system approved for use under section 11 of this rule, designed to interpret and convert individual output signals from pollutant concentration monitors, flow monitors, diluent gas monitors, and other component parts of the monitoring system to produce a continuous record of the measured parameters in the measurement units required by section 11 of this rule.

  • Predictive emissions monitoring system or "PEMS" means all of the equipment necessary to monitor process and control device operational parameters (for example, control device secondary voltages and electric currents) and other information (for example, gas flow rate, O2 or CO2 concentrations), and calculate and record the mass emissions rate (for example, pounds per hour) on a continuous basis.

  • Dielectric material means a material that does not conduct direct electrical current. Dielectric coatings are used to electrically isolate UST systems from the surrounding soils. Dielectric bushings are used to electrically isolate portions of the UST system (e.g., tank from piping).

  • Biodegradable means degradable through a process by which fungi or bacteria secrete enzymes to convert a complex molecular structure to simple gasses and organic compounds.

  • Wedge filter means a filter which effects continuous change in transmission over all or a part of the useful beam.

  • Snow tyre means a tyre whose tread pattern, tread compound or structure is primarily designed to achieve in snow conditions a performance better than that of a normal tyre with regard to its ability to initiate or maintain vehicle motion."

  • Child-resistant packaging means packaging that is designed or constructed to be significantly difficult for children under five years of age to open or obtain a toxic or harmful amount of the substance in the container within a reasonable time and not difficult for normal adults to use properly, but does not mean packaging that all children under five years of age cannot open or obtain a toxic or harmful amount of the substance in the container within a reasonable time.

  • Diatomaceous earth filtration means a process resulting in substantial particulate removal in which a precoat cake of diatomaceous earth filter media is deposited on a support membrane (septum), and while the water is filtered by passing through the cake on the septum, additional filter media known as body feed is continuously added to the feed water to maintain the permeability of the filter cake.

  • Dynamic driving task means the same as that term is defined in Section 41-26-102.1.

  • Cyanoacrylate adhesive means any adhesive with a cyanoacrylate content of at least 95% by weight.

  • Filter profile means a graphical representation of individual filter performance, based on continuous turbidity measurements or total particle counts versus time for an entire filter run, from startup to backwash inclusively, that includes an assessment of filter performance while another filter is being backwashed.

  • Dataset means the data specified in Appendix 1 (Environmental Information) to Schedule 11.2 (Management Information) as the same may be amended from time to time by the Secretary of State (acting reasonably);

  • HEPA filter means a high efficiency particulate absolute air filter capable of trapping and retaining 99.97 percent of fibers greater than 0.3 micrometers in mass median aerodynamic diameter equivalent.

  • Nitrogen oxides means nitric oxide and nitrogen dioxide, expressed as nitrogen dioxide (NO2);

  • Slow sand filtration means a process involving passage of raw water through a bed of sand at low velocity (generally less than 0.4 meters per hour) resulting in substantial particulate removal by physical and biological mechanisms.