Program PARTENERIATE - PNII-PT-PCCA-2011-3.2-0979 Contract nr. 164/2012
Autoritate contractanta:
Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii Dezvoltarii si Inovarii
Contractor principal: Institutul de Xxxxxx Xxxxxxxxxxxxxxx “Xxxxx Xxxx" Iasi
BIONANOMED
Program PARTENERIATE - PNII-PT-PCCA-2011-3.2-0979
Contract nr. 164/2012
Domeniul: Materiale, procese si produse inovative
Durata proiect: 2012 - 2016
CONSORTIU
Coordonator proiect:
INSTITUTUL DE CHIMIE MACROMOLECULARA “XXXXX XXXX” IASI
Director proiect CO: Xx. Xxxxxxxx Xxxxxx
Partener P1: INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU STIINTE BIOLOGICE BUCURESTI (INCDSB)
Director proiect P1: Xx. Xxxxxx Xxxxx
Partener P2:
S.C. I.C.P.E. BISTRITA
S.A. (ICPE) Director proiect P2: Dr. Xxxxx Xxxx
OBIECTIVE GENERALE
▪ Obtinerea unei noi categorii de materiale – BIONANOCOMPOZITE continand amestecuri de polimeri sintetici cu polimeri naturali, optimizate cu nanoparticule de argint cu efect antimicrobian.
▪ Stabilirea unor tehnologii de prelucrare a acestor materiale in vederea obtinerii unor dispozitive medicale cu proprietati speciale, biocompatibile, biodegradabile, antimicrobiene.
OBIECTIVE SPECIFICE
• bioactivarea unor polimeri sintetici biodegradabili nou sintetizati (PLA, PVA, PU) modificati cu polimeri naturali in vederea cresterii biocompatibilitatii;
• obtinerea de nanoparticule de argint cu dimensiuni controlate;
• optimizarea bionanocompozitelor polimerice obtinute prin imprimarea de proprietati antimicrobiene;
• caracterizarea fizico-chimica si biologica a bionanocompozitelor obtinute in vederea stabilirii domeniului lor de utilizare;
• elaborarea unei tehnologii de obtinere a unui dispozitiv
medical biodegradabil cu proprietati antimicrobiene
BUGETUL PROIECTULUI
Valoare de la buget (lei) | Cofinantare proprie (lei) | |
Etapa 2012 | 254 000 | 0 |
Etapa 2013 | 815 100 | 62 103 |
Etapa 2014 | 385 000 | 29 020 |
Etapa 2015 | 381 900 | 31 361 |
Etapa 2016 | 476 500 | 65 016 |
Total | 2 312 500 | 187 500 |
Total proiect | 2 500 000 | |
ETAPA I (26.07.2012 – 10.12.2012):
Stabilirea/selectarea conditiilor din punct de vedere stiintific si tehnic pentru obtinerea unor materiale cu proprietati antimicrobiene
Activitati:
▪ Realizarea unui studiu privind obtinerea dispozitivelor medicale biodegradabile si antimicrobiene
Diseminarea rezultatelor: Etapa 2012
Comunicari
2eme Colloque Franco-Roumain de Chimie Medicinale, Iasi, 3 – 5 Octombrie 2012
1. In vivo and in vitro sustained release study of theophylline from biodegradable and biocompatible alginate/PNIPAAm hydrogels, R. P. Dumitriu, A.M. Oprea, C. N. Xxxxxxxx, X. Xxxxx, C. M. Xxxxxxx, X. Xxxxxxx, X. Xxxxxx
2. Anhydride-modified collagen, X.Xxxxxx, X.Xxxxxx, X.Xxxxxxxxx, X.X Xxxxxx
Zilele Universității ”Al. X. Xxxx”, Conferința Facultății de Chimie, Iași, România, 25-27
Octombrie 2012
3. (Bio)active Layers Deposition by Electrospraying, X. Xxxxxxx, B. S. Xxxxxxxx, X. Xxxxxxx, X.
X. Xxxxxx M. C. Xxxxxx, X. Xxxxxx.
4. Electrospun coatings for antioxidant food packaging, R.P. Xxxxxxxx, X.X. Xxxxxxxx, F. J. Xxxxx,
X. Xxxxxx
5. Hydrolytic degradation of some poly(vinyl alcohol) copolymers for biomedical applications,
R. Lipsa, X. Xxxxxxxxx, X. Xxxxxx, X. X. Xxxxxx, X.Xxxxxxxx
Postere
COST Action FA0904 International Workshop , “Processing technology and functional properties of polymer nanomaterials for food packaging”, Wroclaw, Poland Sept 11-12, 2012
1. Electrospraying of the Chitosan/Vitamin E Formulations onto Various Substrates, B. S. Xxxxxxxx, X. Xxxxxxx, X. Xxxxxx, M. C. Xxxxxx, X. Xxxxxx
Zilele Universității ”Al. X. Xxxx”, Conferința Facultății de Chimie, Iași, România, 25-27 Octombrie (2012).
2.Testing of antioxidative and antimicrobial activity of some new food packagings, X. Xxxxxxx,
X. Xxxxxx, R.N. Darie, X. Xxxxxxx, B.S. Munteanu.
3. Gas permeability of some biocomposites; X. Xxxxxxx, X.X. Xxxxx, A. Xxxxxxxxxxxx, X. Xxxxxx
NanoTech Italy 2012 International showcase for nanotechnologies, Workshop COST Action:
FA0904, Safety, Ethics and Societal Impact Venetia, Italia, 23 noiembrie 2012
4. Toxicity, biocompatibility and degradability of some modified natural polymers used for food packaging, R.P. Dumitriu, A.M. Oprea, X. Xxxxxx
ETAPA II (11.12.2012 – 4.12.2013):
Obtinerea unor nanocompozite polimerice biocompatibile si biodegradabile cu proprietati antimicrobiene
Activitati:
▪ Sinteza si caracterizarea unor polimeri biodegradabili comerciali si sintetizati in laborator: alcool polivinilic (PVA), poliuretan (PU) si acid polilactic (PLA)
▪ Prepararea unor materiale bionanocompozite pe baza polimerilor obtinuti si a
unor biopolimeri naturali: colagen, elastina, condroitin sulfat, acid hialuronic
▪ Obtinerea de nanoparticole de argint prin metoda electrolizei cu electrozi de argint precum si prin metoda electrospinning
▪ Studiul interactiunii dintre amestecurile bioactive si nanoparticule in scopul obtinerii unor materiale cu proprietati antimicrobiene
▪ Evaluarea caracteristicilor fizico-chimice ale bionanocompozitelor bioactive si
antimicrobiene obtinute
▪ Evaluarea in vitro a biocompatibilitatii materialelor prin determinari fizico chimice (conform Farmacopeei Europene in vigoare) si biologice prin determinarea citotoxicitatii
Rezultate stiintifice obtinute
S-au preparat si caracterizat bionanocompozite si componentii lor:
– S-a perfectionat instalatia de “electrospinning” si s-au obtinut
nanoparticule de Ag (AgNP), Ag/chitosan
– Poliuretan/biopolimeri/nanoparticule de argint prin incorporare sau prin depunere
– Acid polilactic/nanoparticule de argint/Vitamina E; acid polilactic/chitosan si acid polilactic/chitosan/ (AgNP),
– Poli(vinil alcool)/chitosan
Caracterizarea s-a efectuat prin spectroscopie UV, FT-IR, reologie, SEM, AFM, XDR, studiul proprietatilor mecanice si termice, permeabilitate la gaze, proprietati antioxidante, titrari potentiometrice,
S-au realizat unele teste microbiologice, de activitate antimicrobiana si antioxidanta
Diseminarea rezultatelor: Etapa 2013
Publicatii:
1. Preparation of medical devices with antimicrobial properties, M. Râpă, X. Xxxxxx, E. E. Tănase, X. Xxxxx, X. Xxxx, Journal of Optoelectronics and Advanced Materials Vol. 15, No. 7- 8, July – August 2013, P. 807 – 816;
2. Novel Environmentally Friendly Copolymers Carboxymethyl Starch Grafted Poly(Lactic Acid) Rodica Lipsa. Xxxx Xxxxxxxxx, Xxxxxxxx Xxxxxx, Xxxxxx Xxxxxxx, Xxxx Xxxxxxxx, X Xxxxx Xxxxxxx (2013) 21:461–471;
3. Evaluation of the Controlled Release Ability of Theophylline from Xanthan/Chondroitin Sulfate Hydrogels. Xxx-Xxxxx Xxxxx, Xxxxxxx-Xxxxxxx Xxxxxx, Xxxxxx Xxxxx Xxxx, Xxxxxxxx Xxxxx Xxxxxxxx, Xxxxxxxx Xxxxxx Journal of Biomaterials and Nanobiotechnology, 2013, 4,
***-*** Published Online April 2013 (xxxx://xxx.xxxxx.xxx/xxxxxxx/xxxx)
4. Lignin Modification with Carboxylic Acids and Butyrolactone under Cold Plasma Conditions, Xxxx Xxxxxxx, Xxxxxx Xxxxxxx, Xxxxxxxx Xxxxxx, Xxxxxx Xxxxxxxx, and Xxxxxxxx Xxxxxx, xx.xxx.xxx/00.0000/xx0000000, Ind. Eng. Chem. Res. 52, 13264–13271 (2013)
Publicatii (continuare):
5. Chitosan coatings applied to polyethylene surface to obtain food-packaging materials. Xxxxxxxx Xxxxxxxxx Xxxxxxxx, Xxxxx Xxxxxxx, Xxxxxx Xxxx Xxxxxxx, Xxxxxxxx Xxxxxxx, Xxxx Xxxxxxx Xxxxxxx, Xxxxxxxx Xxxxxx, Xxxx. Chem. Technol. 48 (5-6), 565-575 (2014);
6. Effectiveness of chitosan as antimicrobial agent in LDPE/CS composite films as poultry minced meat packaging materials, X. Xxxxxx, X.X. Xxxxx, X. Xxxxxxx, X. Xxxxxxx, A. Xxxxxxxxxxxx, X. Xxxxxxx, S.B. Xxxxxxxx, X. Zuburtikudis, Cell. Chem. Technol 48 (3-4), 325- 336 (2014);
Conferinte:
COST ACTION FA0904 “Eco-sustainable Food Packagingbased on Polymer Nanomaterials” INTERNATIONAL WORKSHOP: Characterization, Mechanics and Performance of Innovative Polymer Nanomaterials for Food Packaging Application 24-25 September 2013, Varna, Bulgaria. Xxxxxxxx Xxxxxx-Xxxxx” Hotel Congress Complex, “Sts. Xxxxxxxxxxx and Xxxxxx”, Varna
1. Poly(vinyl alcohol) composites and nanocomposites for food packaging. X. Xxxxxx, X. Xxxxxxxxx
X. Xxxxxx;
2. Characterization by AFM and nano-indentation method of some stratified nanocomposites destined to food packaging. X. Xxxxxxx,X. Xxxxxx,X. Xxxxxxxx, X. Xxxxxxxxxx, X. Xxxxxx
3. New potential applications in food packaging of the electrospinning / electrospraying method used for polymer surface modification and to obtain of nano(micro)structures from antimicrobial agents as chitosan, silver and various bioactive agents. I. Polylactic acid / Silver NP/Vitamin E bio-nanocomposite fibers with antibacterial and antioxidant activity. X. Xxxxxxxx, X. Xxxxx, X. Xxxx, X. Xxxxxx.
Comunicari:
10th International Conference on Nanosciences & Nanotechnologies (NN13) 9- 12 July 2013, Thessaloniki, Greece
1. Poly(vinylalcohol)/chitosan/montmorillonite hydrogel nanocomposites, Xxxxx Xxxxxxxxx, Xxxxxxxx Xxxxxxxx Xxxxxx, Xxxxxxx-Xxxxxxx Xxxxxx, Xxxxxxxx Xxxxxx
2. Polyethylene/ chitosan-Na+-MMT nanocomposites, R.N. Darie, X. Xxxxxxx, X. Xxxxxxx, A. Xxxxxxxxxxxx, X. Xxxxxxxxxx, X. Xxxxxxxxxxxx, X. Fras, X. Xxxxxx, X. Xxxxxx
Al XI-lea Simpozion Internaţional de Produse Cosmetice şi Aromatizante, Iaşi –
Romania, 4 – 7 Iunie 2013
3.“Textile de uz cosmetic cu caracter antioxidant”, Xxxxxxxx Xxxxxxx, Xxxxxx Xxxxxxx, Xxxxxxxx Xxxxxx Xxxxxxx Xxxxxxxx, Xxxxxx X. Xxxxxxx, Xxxxxx Xxxxxxxx
4. Biomateriale pe baza de nanocompozite alcool polivinilic/chitosan / montmorillonit preparate prin metoda inghet dezghet X. Xxxxxxxxx, C. Xxxxxxxx Xxxxxx, X.-X. Xxxxxxxx, X. Xxxxxx
Comunicari (continuare):
Zilele Academice Iesene, A XXIV-a Sesiune de Comunicari Stiintifice a Institutului De Chimie Macromoleculara „Xxxxx Xxxx” Iasi:Progrese în Stiinta Compusilor Organici și Macromoleculari Iasi, 3 - 5 Octombrie 2013
5. Caracterizarea Termica a Retelelor Semiinterpenetrate Pe Baza De Colagen Si 2-
Hidroxietil Metacrilat X. Xxxxxx, X. Xxxxxx, X. Xxxxxx.
6. Depunerea de Formulari Biocompatibile pe Substrat Poliuretanic prin Electrospraying / Electrospinning. B. S. Xxxxxxxx, X. Xxxxxxxxxxx, X. Xxxxxxx, X. Xxxxxx, X. Xxxxxxxx, X. Xxxxxx
7. Activarea cu Enzime pentru Obtinerea de Materiale Textile Inteligente. X. Xxxxxxx, X.Xxxxxxx, X. Xxxxxxxx, X. Xxxxxx.
8. Imobilizarea Imunoglobulinei G si a Fibrinogenului pe Suprafata Poli(Fluorurii de
Viniliden) X. Xxxxxxx, X. X. Xxxxxx, M. C. Xxxxxx, X. Xxxxxxx, X. Xxxxxx, X. Xxxxxx.
Postere:
The 8th International Conference on Materials Science & Engineering – BraMat 2013, BRASOV, 28 Febr.-2 March 2013
1.Preparation of medical devices with antimicrobial properties. M. Râpă, X. Xxxxxx,X. Xxxxxx, X. Xxxxx, X. Xxxx
International Workshop Waedenswil, Switzerland, March 21-22, 2013 COST FA0904:Eco-sustainable food packaging based on polymer nanomaterials: “Development of new safe PNFP and Development of strategies to identify any critical interaction of PNFP with food”
2. Polyethylene - chitosan composites and nanocomposites for food packaging X. Xxxxxx, X.
Xxxxx, X. Xxxxxxx, X. Xxxxxxxx-Xxxxxx, X. Zuburtikudis, Baklavaridis A.,G-M.Pricope.
10th International Conference on Nanosciences & Nanotechnologies (NN13) 9-12 July 2013, Thessaloniki, Greece
3. Hybrid collagen-based hydrogels with embedded montmorillonite nanoparticles X.X. Xxxxxx,
X. Xxxxxx, X.X. Xxxxxxx
4. Biocompatibility, biodegradability and drug carrier ability of the hybrid collagen-based hydrogel nanocomposites. X. X. Xxxxxx, X. Xxxxxx, X. X.Xxxxxxx, X. Xxxxxx;
5. Effect of nanoclay hydrophibicity on the PLA/clay nanocomposites properties. Xxxxxxx, X. Xxxxx, X. Xxxxxxx, X. Xxxxxxxxxxxx, R.P. Xxxxxxxx, X. Xxxxxxxxxx, X. Zuburtikudis A., X. Xxxxxx
Postere (continuare):
Salonul Naţional de Inventică CHIM - INVENT 2013, Iaşi – Romania, 3 – 5 iulie 2013
6. Obţinerea fibrelor textile receptive la modificări de temperatură şi pH, X. Xxxxxxx, X.X. Xxxxxx, X. Xxxxxx, a primit diploma si medalia “Xxxxx Xxxx” iar X. Xxxxxxx a primit diploma si cupa “Tanarul cercetator”.
7. Procedeu de obtinere a unui hidrogel bicomponent , R.P. Xxxxxxxx, X. Xxxxxx, a primit diploma si medalia “Xxxxx Xxxx”.
Zilele Academice Iesene, A XXIV-a Sesiune de Comunicari Stiintifice s Institutului De Chimie Macromoleculara „Xxxxx Xxxx” Iasi:Progrese în Stiinta Compusilor Organici și Macromoleculari Iasi, 3 - 5 Octombrie 2013
8. Evaluarea Eficientei Analgezice A Ketoprofenului Încorporat in Hidrogeluri de Poli(N- Izopropilacrilamida) / Alginat de Sodiu. X. Xxxxxxx, X- X. Xxxxxxxx, X.Xxxxxx;
9. Membrane Biocompatibile de Baza de Poliuretan Continând Diferiti Compusi Naturali si
Nanoparticule de Argint. X. Xxxxxxx, X. Xxxxxxxxxxx, X. Xxxxx, X. Xxxxxxx, S. B. Xxxxxxxx,
X. Xxxxx, X.Xxxxxxxxxx, X. Xxxxxxx, X. Xxxxxx.
Etapa III. (5.12.2013 – 4.12.2014):
Evaluarea si optimizarea caracteristicilor compozitelor obtinute prin amestecarea polimerilor naturali si sintetici si a nanomaterialelor in diverse combinatii
Activitati:
• Studiul interactiunii dintre amestecurile bioactive si nanoparticule in scopul obtinerii unor materiale cu proprietati antimicrobiene;
• Evaluarea caracteristicilor fizico-chimice ale bionanocompozitelor bioactive si
antimicrobiene obtinute;
• Evaluarea prelucrabilitatii materialelor - Caracteristici, determinari, capacitati (laboratoare acreditate, etc.);
• Evaluarea stabilitatii termice a materialelor;
• Evaluarea in vitro a biocompatibilitatii materialelor prin determinari fizico chimice (conform Farmacopeei Europene in vigoare) si biologice prin determinarea citotoxicitatii ;
• Evaluarea activitatii antimicrobiene a materialelor cu ajutorul antibiogramei,
prin metoda difuzimetrica Kirby – Bauer adaptata ;
• Evaluarea degradabilitatii in diferite medii biologice;
• Selectarea nanocompozitelor la care raportul prelucrabilitate/ biocompatibilitate/ biodegradabilitate/ proprietati antimicrobiene este optim.
Rezultate stiintifice obtinute
• S-au dezvoltat noi nanocompozite la care raportul prelucrabilitate/ biocompatibilitate/ biodegradabilitate/ proprietati antimicrobiene este optim:
– Bionanocompozite cu matrice poliuretanică prin metoda evaporării
solventului si electrospinning;
– Bionanocompozite pe bază de acid polilactic(PLA)/ulei epoxidat
/nanoparticule de Ag (Ag NP) sau chitosan și agenți antioxidanți
prin prelucrare in topitura si electrospinning;
– Bionanocompozite pe bază de PLA/PCL/AgNPs și PLA/policaprolactonă (PCL)/Alg/Zn prin prelucrare in topitura;
– Bionanocompozite pe bază de poli(alcool vinilic) și nanoargile.
– S-au efectuat teste preliminare de degradare in mediu pentru copolimeri conținând poli(alcool vinilic) si acid polilactic sau aspartic
Publicaţii:
Diseminarea rezultatelor: Etapa 2014
1. Effect of Nanoclay Hydrophilicity on the PLA/Clay Nanocomposites Properties, R.N. Darie, X. Xxxxxxx, X. Xxxxxxx, G. M. Pricope, X. X.Xxxxxx, X. Xxxxxx, X. Xxxxxxxxxxxx, X. Xxxxxxxxxxxx, X.Xxxxxx, Industrial & Engineering Chemistry Research, 53 (19), 7877–7890 (2014).
2. Poly(lactic acid) co-Aspartic acid copolymers, Possible utilization in drug delivery systems,
Xxxxxxxxx X., Xxxxx R., Xxxxxx X., X. Xxxxxxx, X. Polym. Env., 21:1064–1071 (2013).
3. Biocompatible and biodegradable alginate/poly(N-isopropylacrylamide) hydrogels for sustained theophylline release, R. P.Xxxxxxxx, X.-X. Xxxxx, C.N. Xxxxxxxx, X.-X. Xxxxxx, X. Xxxxx,
X. X. Xxxxxxxx, X. Xxxxxxx, X.Xxxxxx, X. Appl. Polym. Sci., 131(17), 40733-40748 (2014).
4. Polylactic acid (PLA)/Silver-NP/VitaminE bionanocomposite electrospun nanofibers with antibacterial and antioxidant activity, B. S. Xxxxxxxx, X. Xxxxx, X.X. Pricope, X.Xxxx, X. Xxxxxx, X. Nanoparticle Research, in press
5. Polyurethane-extracellular matrix silver bionanocomposites for urinary catheters, X.Xxxxxxxxxxx, X.Xxxxx, X. Xxxxxxx, B. S. Xxxxxxxx, X. X. Xxxxxxxx, X. X. Pricope, X. Xxxxxx, X. Xxxxxxxx, X. Xxxx and X.Xxxxxx, X. Bioact. Compat. Polym. Accepted
6. Polyurethane biocompatible silver bionanocomposites for biomedical applications. X. Xxxxx, X. Xxxxxxxxxxx, X. Xxxxxxx, B. S. Xxxxxxxx, X. X. Xxxxxxxx, X. Xxxxx and X.Xxxxxx X. Nanoparticle Research, 10.1007/s11051-014-2710-x
7. Degradation of polyvinyl alcohol)-graft-lactic acid copolymers by fungus Trichotecium Roseum.
R. Lipșa, Xxxx Xxxxxxxxx, V.C. Xxxxxxxx, X. Xxxxxx, X. Appl. Polym. Sci, accepted
8. Polyvinyl alcohol and starch blends: properties and biodegradation behaviour., M. Xxxx, X. Xxxxx, X. Xxxxxx, X. Xxxxxxxx, X. Xxxxxxx, Ecoterra, Journal of Environmental Research and Protection, 2014, 11(1):34-42.xxxx://xxx.xxxxxxxx-xxxxxx.xx/xxxxx/0000000000.xxx.
Cărţi:
1. Xxxxxxx Xxxxxx, Xxxxxxxx Xxxxxx: Aplicaţiile medicale şi farmaceutice ale unor biomateriale şi biocompozite sensibile la stimuli externi, Technopress, 2014, ISBN: 978-606-687-149-5
Capitole de carte:
1. Drug delivery and Release from polymeric nanomaterials, X. Xxxxxx, X.X. Oprea,
X.X. Xxxxxx, A.M. Cojocariu, Chap. 2 in Nanotechnology and Drug Delivery, Ed. X.X. Xxxxx,
Vol.1, 2014, CRC Press, Xxxxxx and Xxxxxxx Group, p. 28-81.
2. Design and Mechanical Performance: Polyolefin / Biomass Composites, X. Xxxxxx, X. X. Xxxxx, X. Xxxxxxxxx, Xxxxxxxx Publishing (Elsevier) Chapter 16 in Biocomposites: Design and Mechanical Performance Eds. Xxxxxxxx Xxxxx , Xxxx Xxxxx Xxxxxxx, Xxxxxxxx Xxxxx Xxxxxx, Xxxx Xxxxx, Xxxxxxxx (Elsevier) Publishing : in press
Cereri brevete depuse:
1. Procedeu și compoziție pentru obținerea de noi bionanocompozite polimerice biocompatibile, cu proprietăți antimicrobiene și antioxidante pentru uz medical,
X.X. Xxxxx, X. Lipșa, X. Xxxxxxxxx, X. Xxxxxxx, X. Xxxxxx, X.X. Xxxxxxxx, A/00598/Aug. 2014;
2. Procedeu și compoziție de obținere prin electrofilare / electropulverizare a unor suprafețe poliuretanice bioactive (biocompatibile și antimicrobiene) pentru uz medical și farmaceutic”, B.S. Xxxxxxxx, X. Xxxxxxx (Xxxxxxx), X. Xxxxxxxx, X. Xxxxx, X. Xxxxxx, A/00651/Aug. 2014.
Comunicări:
1. Antibacterial membranes based on polyurethane and biological polymers, B.S. Xxxxxxxx, X. Xxxxxxxxxxx, X. Xxxxxxx, R. P. Xxxxxxxx, X.X. Xxxxxx, Xxxxxxxx Xxxxxx, International Conference "Eco-sustainable Food Packaging Based on Polymer Nanomaterials" COST ACTION FA0904, 26-28 February 2014, Roma, Italia.
2. Evaluarea in vitro a hidrogelurilor pe bază de poli (alcool vinilic)/chitosan ca sisteme cu eliberare controlată a bisoprololului fumarat. The in vitro testing of polyvinyl alcohol/chitosan hydrogels as systems for controlled delivery of bisoprolol fumarate Autori: X. Xxxxxx, X. Xxxxxxxxx, C.N. Xxxxxx, X. Xxxxxx X. Xxxxxx, Zilele Universităţii,"Xxxxxxxxx Xxxx Xxxx” din Iaşi, Conferinţa Facultăţii De Chimie & 3ème Colloque Franco-Roumain de Xxxxxx Xxxxxxxxxx, Ediţia 2014, Iasi, 31 oct – 01. nov., 2014, xxxx://xxx.xxxx.xxxx.xx/xx/xxxxxxxxxxx/
3. Poly(vinyl alcohol) copolymers biodegradation with Trichotecium roseum fungi, R. Lipsa, X. Xxxxxxxxx, X. Xxxxxxxx, X. Xxxxxx, X. Xxxxxxxxxx, A. P. Xxxxxxx, X. Xxxxxxx, Zilele Universităţii,"Xxxxxxxxx Xxxx Xxxx” din Iaşi, Conferinţa Facultăţii De Chimie & 3ème Colloque Franco-Roumain de Xxxxxx Xxxxxxxxxx, Ediţia 2014, Iasi, 31 oct – 01. nov., 2014, xxxx://xxx.xxxx.xxxx.xx/xx/xxxxxxxxxxx/
Postere:
1. Electrospraying of chitosan/vitamin E mixtures for bioactive coatings, R.P. Dumitriu, B.S. Xxxxxxxx, X. Xxxxxxx, X. Xxxxxx, International Conference on Green Chemistry and Sustainable Engineering Barcelona, 29-31 July 2014
2. Polyurethane/biopolymers films and electrospun fibrous coatings for antimicrobial materials, R.P. Xxxxxxxx, X. Xxxxxxx, B.S. Xxxxxxxx, X. Xxxxxxxxxxx, X. Xxxxx, X.X. Xxxxxx,
X. Xxxxx, X. Xxxxxx, International Conference on Green Chemistry and Sustainable
Engineering Barcelona, 29-31 July 2014
3. Smart textiles obtained by enzymatic activation followed by surface grafting of N- isopropylacrylamide, X. Xxxxxxx, X. Xxxxxxx, X. Xxxxxxxx, X. Xxxxxxxx, X. Xxxxxx, International Conference on Green Chemistry and Sustainable Engineering, 29 - 31 July 2014, Barcelona, Spain;
4. Effect of Poly(ε-caprolactone)/Chitosan ratio on the properties of the Electrospun Nanofibres, X. Xxxxxxx, R.P. Xxxxxxxx, X. Xxxxxxxxxxxx, X. Xxxxxx, International Conference on Green Chemistry and Sustainable Engineering, 29 - 31 July 2014, Barcelona, Spain.
5. Protein nanolayer with antimicrobial and antioxidant properties immobilized onto cold plasma or gamma activated poly(lactic acid) substrate, E. Xxxxxxx Xxxxxxx, X.X. Xxxxxx,
X.X. Xxxxxx, X. Xxxxxxxxx, X. Xxxxxxx, G. M. Pricope, X. Xxxxxx, 6th Szeged International Workshop on Advances in Nanosciences, 15-18 Octombrie, Szeged, Ungaria;
6. Method and composition for obtaining stratified composites, X. Xxxxxxx, B.S. Xxxxxxxx,
R.P. Xxxxxxxx, X. Xxxxxx, The XVIII-th International Exhibition of Inventics, Research and
Technological Transfer "INVENTICA 2014" , July 2nd – 4th, 2014, Iasi, Romania;
7. Poly(vinylidene fluoride) surface modified with fibrinogen – Scaffold for cell culture, X. Xxxxxxx, X. Xxxxx, X. Xxxxxx, X. Xxxxxx, Joint IUBMB/ICGEB Symposium on Modern Biotechnological Advances for Human Health – BAHH, May 20-22, 2014, Bucharest, Romania;
Postere (continuare):
8. Effect of silver nanoparticles on the dispersion, rheological properties and morphological aspect of solvent cast polyurethane/biopolymers bionanocomposite membranes – R.P. Xxxxxxxx, X. Xxxxxxxxxxx, X. Xxxxx, X. Xxxxxx (P31), 6th International Conference "Biomaterials, Tissue Engineering & Medical Devices" BiomMedD’2014, 17-20 Septembrie 2014, Constanta, Romania;
9. Preparation And Characterisation Of Poly(Lactic Acid)/Poly(Ε-Caprolactone)/Sylver Nanoparticles Bionanocomposites, X. Xxxxxx, X. Xxxx, X. Xxxxx, X.-X. Xxxxxxxxx, X. Xxxxx,
R. Xxxxx, X. Xxxxxx, X. Xxxxx, Simpozionul International PRIOCHEM, Prioritatile Chimiei Pentru o Dezvoltare Durabila, Ed. A X-A, ICECHIM, Bucuresti, 30-31 Octombrie 2014 (Poster).
10. Novel Bioactive And Biodegradable Materials For Medical Applications, X. Xxxxxx, X. Xxxx, X.-X. Xxxxxxxxx, X. Xxxxx, X. Xxxxx, X. Xxxxxx, Gr. Xxxx, X. Xxxxx, The 10th Environmental Legislation,Safety Engineering And Disaster Management (Elsedima) International Conference, 18-19 Septembrie 2014, Cluj-Napoca, Isbn: 978-973-606-526-183-9 (Poster).
11. Biocompatibility Of New Biomaterials Based On Polyvinyl Alcohol And Natural Compounds With Silver Nanoparticles, International Symposium On Modern Biotechnological Advances For Human Health – Bahh X. Xxxxx, X. Xxxxxx, X. Xxxxx, X. Xxxxxx, X. Xxxx, X. Xxxxxxxx, May 20-22, 2014, Bucharest, Romania (Poster).
12. Novel Biopolymeric Composites With Anti-Microbial And Anti-Biofilm Properties Based On Polycaprolactone, Chitosan and Essential Oils, X. Xxxxxx, M. C. Xxxxxxxxx, X.Râpă, X. Xxxxxx,
X. Xxxxx, The 24th European Congress Of Clinical Microbiology And Infectious Diseases (Eccmid), Barcelona, Spania, 10-13 Mai 2014. (Poster P0321, Conferinţă Internaţională Cotată ISI).
Teze de doctorat susţinute:
1. Xxxxx Xxxxxxx: Elaborarea de suprafeţe polimerice receptive la factori externi – 24 Ianuarie 2014
2. Xxxxx Xxxxxxxxx: Contribuții la obținerea și caracterizarea unor noi materiale polimerice degradabile in mediu – 27 iunie 2014
Etapa IV. (5.12.2014 – 4.12.2015)
Evaluarea fizico-chimica si biologica a compozitelor selectate in etapa III
Activitati:
• Evaluarea in vitro a biodegradabilitatii compozitelor selectionate in mediu biologic simulat;
• Selectarea celor mai bune formulari ale bionanocompozitelor pe baza de PLA, PVA si PU si agenti antimicrobieni (teste de eliberarea a agentului bioactiv) si investigarea proprietatilor de suprafata;
• Testarea in vitro a citotoxicitatii bionanocompozitelor prin metode directe si indirecte;
• Evaluarea activitatii antimicrobiene;
• Evaluarea in vitro a biocompatibilitatii produselor;
• Determinarea efectului materialelor studiate asupra gradului de viabilitate si a proliferarii celulare
Rezultate stiintifice obtinute
❖ Corelarea comportarii mecanice, termice si reologice cu aspectele morfologice ale bionanocompozitelor avand diferite continuturi de nanoparticule de argint pentru a stabili concentratia minima a acestora care asigura caracterul antimicrobian fara a afecta biocompatibilitatea.
❖ Evaluarea in vitro a biodegradabilitatii compozitelor selectionate in mediu biologic simulat. S-a efectuat evaluarea pentru compozite cu cele trei matrici PLA, PU, PVA:
• Degradarea hidrolitica in solutii de diferite pH-uri
• Degradarea enzimatica in prezenta enzimelor secretate de Trichoderma viride
• Actiunea degradativa a fungului Phanerochaete chrysosporium
❖ Evaluarea activitatii antimicrobiene:
• bacterii Gram-pozitive: Staphylococcus aureus ATCC 29213, Staphylococcus aureus, Staphylococcus xylosus, Enterococcus fecalis
• bacterii Gram-negative: Escherichia coli; Escherichia coli, Klebsiella pneumoniae
• microfungi: Candida albicans ATCC, Listeria monocytogenes si Salmonella typhymurium.
❖ Evaluarea in vitro a biocompatibilitatii produselor
– gradului de viabilitate si de proliferare celulara
– Imbunatatirea metodei citometrie de flux
• determinarea ADN total,
• a continutului de colagen,
• evaluarea proprietatilor de suprafata.
❑ S-a stabilit ca toate bionanocompozitele sunt biocomatibile iar eliberarea agentilor bioactivi are loc controlat/prelungit prin mecanisme ne-Fickiene si dupa un timp indelungat prin degradarea matricei.
ETAPA IV. (5.12.2015 – 4.12.2016)
Evaluarea biologica a compozitelor selectate si obtinerea unui produs experimental (tub de drenaj urinar cu actiune antimicrobiana) pe baza compozitelor obtinute
REZULTATE OBTINUTE:
Elaborarea tehnologiei de extrudare la faza miropilot pentru obtinerea unui dispozitiv medical (tub de drenaj urinar)
Rezultate stiintifice obtinute
Bionanocompozitele selectate in fazele anterioare ale proiectului au fost obtinute prin tehnologia micropilot elaborata si au fost detaliat caracterizate stabilindu-se:
⮚biocompatibilitatea
⮚gradul de viabilitate
⮚proliferarea celulara prin citometrie de flux
⮚continutul de DNA total
⮚stabilirea influentei materialelor asupra ciclului celular si evaluarea imuno-histo- chimica asupra sintezei componentilor matricei extracelulare respectiv a colagenului,
⮚evaluarea activitatii antimicrobiana prin studierea biofilmului depus pe suprafata
acestuia,
⮚toxicitatea in vivo si
⮚testarea preclinica a tubului de drenaj urinar.
❑ REZULTATELE AU ATESTAT FAPTUL CA BIOCOMPOZITELE OBTINUTE INDEPLINESC CERINTELE STANDARDIZATE IMPUSE PENTRU TUBURILE DE DRENAJ URINAR
Diseminarea rezultatelor: Etapa 2016
Publicatii
1. New antimicrobial chitosan derivatives for wound dressing applications, Xxxx Xxxxx Xxxxxxxxx, Xxxxxxx Xxxxxxx Xxxxx, Xxxxxx Xxxx, Xxxxxxxxxx Xxxxxxx, Xxxxxxx Xxxxxxxx, Xxxxxxxx Xxxxxxxx, Xxxxxxx Xxxxx, Xxxxx Xxxxxx, Xxxxx Xxxxxxx, Xxxxxx Xxxxxx, Xxxxxxxx Xxxxxx, Xxxxxx Xxxxx, Xxxxxx Xxxxxxx, Carbohydrate Polymers 141 28–40 (2016);
2. Complex poly(lactic acid)-based biomaterial for urinary catheters: I. Influence of AgNP on properties, Xxxxxx X. Xxxxx-Xxxx, Xxxxxx X. Xxxxxxxx, Xxxx Xxxxxxxxx, Xxxxxx Xxxxx, Xxxxx Xxxxxxx, Xxxxxxx Xxxxxxxx, Xxxxxxxx Xxxxxx, Bioinspired, Biomimetic and Nanobiomaterials DOI: xxxx://xx.xxx.xxx/00.0000/xxxxx.00.00000, Published Online: May 18, 2016;
3. Complex poly(lactic acid)-based biomaterial for urinary catheters: II. Biocompatibility, Xxxxx Xxxxxxx, Xxxxxxxx X. Xxxxxxxx, Xxxxxx X. Xxxxx-Xxxx, Xxxx X. Xxxxxxx, Xxxxx Xxxxx, Xxxxxxxx Xxxxxx, Xxxxx Xxxx, Rodica D. Xxxxx, Xxxxxxxx Xxxxxx, Bioinspired, Biomimetic and Nanobiomaterials, xxxx://xx.xxx.xxx/00.0000/xxxxx.00.00000, Published Online:April 07, 2016;
4. Biodegradation of poly(lactic acid) and some of its based systems with Trichoderma viride, Xxxxxx Xxxxx, Xxxx Xxxxxxxxx, Xxxxxx Xxxxxxxx Xxxxx-Xxxx, Xxxxxxxxxxx Xxxxxx,Xxxxxxxx Xxxxxx, Xxxxxx Xxxxxxx, International Journal of Biological Macromolecules 88 515–526 (2016);
5. Polyethylene Materials with Multifunctional Surface Properties by Electrospraying Chitosan / Vitamin E Formulation Destined to Biomedical and Food Packaging Applications, Xxxxx Xxxxxxx, Xxxxxxxxx X. Xxxxxxxx, Xxxxxx X. Xxxxxxxx, Xxxxx Xxxxxxx, Xxxxxx Xxxxxxx, Xxxxxx Fras Xxxxxxx, Xxxx X. Xxxxxxx, Xxxxxxxx Xxxxxx, Iran Xxxxx X., DOI 10.1007/s13726- 000-0000-0 (2016);
6. Poly (vinyl alcohol)/chitosan cryogels as PH responsive ciprofloxacin carriers, X.Xxxxxx, X. Xxxxxxx, X. Xxxxxx, Journal of Polymer Research, 23:146 (2016);
Publicatii - continuare
7. Novel Bioactive and Biodegradable Materials for Medical Applications, X. Xxxxxx, X. Râpă, M.
C. Xxxxxxxxx, X. Xxxxx, X. Xxxxx, X. Xxxxxx, X. Xxxx, X. Xxxxx, Environmental Engineering and Management Journal, November 2015, Vol.14, No. 11, 2703-2711. (FI = 1,065, Print ISSN: 1582- 9596, eISSN: 1843-3707, Accession Number: WOS:00036909099900022, Tip document: Articol);
8. Hybrid Nanostructures Containing Sulfadiazine Modified Chitosan as Antimicrobial Drug Carriers, B.S. Xxxxxxxx, X.X. Xxxxxxxx, X. Xxxxxxx, X. Xxxxxxxxx, G. E. Hitruc, X. Xxxxxxx, X. Xxxxxxxx,
A. L. Matricala, X. Xxxxxx, Xxxxxxxxxxxxx, 6, 2016, xxxx://xxx.xxxx.xxx/xxxxxxx/xxxxxxxxxxxxx;
9. Lactoferrin-immobilized surfaces onto functionalized PLA assisted by the gamma-rays and nitrogen plasma to create materials with multifunctional properties, X. Xxxxxxx, X. Xxxxxxxxx, X. X. Xxxxxx, A. Xxxxx, X. X. Xxxxxx, X. Xxxxxxx, X. Xxxxxxx, X. Xxxxxxx, X.Xxxxx, X.Xxxxxx,
X. Xxxxxx, ACS Materials and Interfaces, doi 10.1021/acsami.6b09069 (2016);
10. Environmentally Friendly Polylactic Acid/Modified Lignosulfonate Biocomposites; X. Xxxxxx, X.X. Xxxxx-Xxxx, X. Xxxxxxx, X. Xxxxxxx, X. Xxxxxxxxxx, X.X. Xxxxxxx, X. Xxxxxxxxx, X. Xxxxxx, Journal of Polymers and the Environment, DOI 10.1007/s10924-016-0868-2 (2016);
11. pH-responsive HEMA / citraconic anhydride modified collagen hydrogels as ciprofloxacin carriers for wound dressings, Short title: pH-responsive hydrogels for wound dressings,
X. Xxxxxx, X. Xxxxxx, X. Xxxxxx, X. Xxxxxxxxx, X. Xxxxxx, X. Bioactive and Compatible Polymers, accepted;
12. Alginate/Lignosulfonate Blends With Photoprotective And Antioxidant Properties For Active Packaging Applications, R.P. Xxxxxxxx, X. Xxxxxx, D. S. Xxxxxxxxx, X. Xxxxxx, X. Xxxxxx, X. Polym. Environ. Submitted.
Capitole in carti:
1. Nanogels of natural polymers, X. Xxxxxx, X. Xxxxxx, Chapter 4 in Handbook on “Polymer gels”. Section Polymer gels: Science and fundamentals. Ed. Xxxxx Xxxxx Xxxxxx, accepted
2. Polysaccharide containing gels for pharmaceutical applications, C. N. Xxxxxxxx- Xxxxxx, X. Xxxxxx Xxxxxxxxxx, X. Xxxx, X. Xxxxxx. Chapter 6 in Handbook on “Polymer gels”. Section Polymer gels: Science and fundamentals. Ed. Xxxxx Xxxxx Xxxxxx, accepted
Teze de doctorat sustinute:
Xxxxxx Xxxxxxx: Noi biomateriale receptive la stimuli externi continand proteine, Coordonatori, X. Xxxxxx si X. Xxxxxx, 9 mai 2016
Cereri de Brevete
• Composition and developing method of new modified release aminodarone hydrochloride matrix tablets with prolonged action, X. Xxxxxxxx, X. Xxxxxx, X. Xxxxxxx, X. Xxxxxx, X.X. Xxxxxxx, X. Xxxxxxx, Patent application A/00138-2016, Medalie de bronz.
• Composition and developing method of new modified release aminodarone hydrochloride complexed with hydroxypropyl-beta-cyclodextrin matrix tablets with prolonged action, X. Xxxxxxxx, X. Xxxxxx, X. Xxxxxxx, X. Xxxxxx, X.X. Xxxxxxxx, X. Xxxxxxx, Patent application A/00139/2016. Medalie de argint.
Activitatea V.9 P2. Redactare brevet, tehnologie de obtinere tub de drenaj urinar cu actiune antimicrobiana
Acid polilactic Compatibilizator Colagen hidrolizat
Uscare
Plastifiant
Ambalare, Sterilizare
Oxid de etilena
Amestecare in topitura Granulare
Agent
Extrudare
antimicrobian
DREN URINAR STERILIZAT
Schema fluxului tehnologic de obtinere a drenurilor urinare
Compozite polimerice pentru obținerea unui tub de drenare urinar cu proprietăți antimicrobiene și procedeu de obținere a acestuia; Rapa M., Xxxxx X., Xxxxx X., Xxxxxx X., Xxxxxx X., Xxxxx-Xxxx R. N., Xxxxxxxx R. P., Xxxxxxxxx M. C., Xxxxxx X., numar de inregistrare OSIM A2016/00876 din 22.11.2016 (beneficiar: S.C. I.C.P.E. Bistrita S.A.)
Communicari orale
⮚Evaluation of polylactic acid antioxidant packaging materials containing xxxxxxxx powder, R.N. Darie-Niță, X. Xxxxxxxxx, X. Xxxxxxx, X. Xxxxx, X. Xxxxxx, International Symposium on Modern biotechnologies in sustainable development of the Danube delta, May 31-June 2, 2016, Murighiol, Tulcea, Romania, p.21-23.
⮚Biodegradable PLA/Chitosan blends for medical and food packaging applications, M. Xxxx, X.X. Xxxxxx, X.X. Xxxxx-Xxxx, X.X. Xxxxxxx, X. Xxxxxx, International Symposium on Modern biotechnologies in sustainable development of the Danube delta, May 31-June 2, 2016, Murighiol, Tulcea, Romania, p.31-33.
Postere
1. Composition and developing method of new modified release aminodarone hydrochloride matrix tablets with prolonged action, X. Xxxxxxxx, X. Xxxxxx, X. Xxxxxxx, X. Xxxxxx, X.X. Xxxxxxx, X. Xxxxxxx, Patent application A/00138- 2016, Proceedings of the 8+th Edition of the European exhibition of creativity and innovation, EUROINVENT’2016, Eds. A.V. Sandu,I.G. Sandu, 19-21 may, 2016, Xxxx Xx. StuDIS, 2016, ISBN 978-606-775-212-0, p.326.
2. Composition and developing method of new modified release aminodarone hydrochloride complexed with hydroxypropyl-beta-cyclodextrin matrix tablets with prolonged action, X. Xxxxxxxx, X. Xxxxxx, X. Xxxxxxx, X. Xxxxxx,
O.M. Xxxxxxxx, X. Xxxxxxx, Patent application A/00139/2016, Proceedings of the 8-th Edition of the European exhibition of creativity and innovation, EUROINVENT’2016, Eds. A.V. Sandu,I.G. Sandu, 19-21 may, 2016, Xxxx Xx. StuDIS, 2016, ISBN 978-606-775-212-0, p.325.
3. Influence of silver nanoparticles concentration on the mechanical and thermal properties of poly(lactic acid) based biomaterial for urinary catheters, X. Xxxxxx, Xxxxxx X. Xxxxx-Xxxx , Xxxxxxxx X. Xxxxxxxx, X. Xxxxxxxxx, X. Xxxxxxx, X. Lipșa, International Symposium on Modern biotechnologies in sustainable development of the Danube delta, May 31- June 2, 2016, Murighiol, Tulcea, Romania, p.50 - 51.
4. Biocompatibility, functional properties and degradability of a poly(lactic acid) based biomaterial, X. Xxxxxxx, B.S. Xxxxxxxx, Xxxxxx X. Xxxxx-Niţă , G.M. Pricope, X. Xxxxx, X. Xxxxxx, X. Râpă, X. Xxxxxx, International Symposium on Modern biotechnologies in sustainable development of the Danube delta, May 31-June 2, 2016, Murighiol, Tulces Romania, p.71 - 72.
5. pH sensitivity and biocompatibility of poly(vinyl alcohol)/chitosan cryogels, X. Xxxxxx, X. Xxxxxxx, X. Xxxxx, X. Xxxxxx International Symposium on Modern biotechnologies in sustainable development of the Danube delta, May 31-June 2 2016, Murighiol, Tulces, Romania, p.73 - 74.
6. Effect of silver nanoparticles on the properties of pla based bionanocomposites for the medical devices field, M. Râpă,
X. Xxxxx, X. Xxxxxx, X. Xxxxxxx, X. Xxxxx, X. Xxxxx, X.-X. Xxxxxxxx, X.-X. Xxxxxxxxx, X. Xxxxxxxxx, X. Xxxxxx The XXXIV-th Roumanian Chemistry Conference, OLTCHIM, Ramnicu Valcea, October 04-07, 2016, Calimanesti-Caciulata, Valcea, Romania
7. Process and composition for producing novel biocompatible polymeric bionanocomposites having antimicrobial and oxidation preventing properties for medical use. Xxxxxx X. Xxxxx-Xxxx , Xxxxxx Xxxxx, Xxxx Xxxxxxxxx, Xxxxx Xxxxxxx, Xxxxxxxx Xxxxxx, Xxxxxxxx X. Xxxxxxxx, INVENTICA 2016, June, 29 – July, 1, Iasi, Romania, diploma și medalie de aur.
8. Effect of silver nanoparticles on the properties of pla based bionanocomposites for the medical devices field, M. Râpă,
X. Xxxxx, X. Xxxxxx, X. Xxxxxxx, X. Xxxxx, X. Xxxxx, X.-X. Xxxxxxxx, X.-X. Xxxxxxxxx, X. Xxxxxxxxx, X. Xxxxxx, A XXXIV-a Conferinta Nationala de Chimie organizata de Centrul de Cercetare Oltchim, Academia Romana – Sectia de stiinte Chimice si Societatea de Chimie din Romania, in perioada 04-07 octombrie 2016, (xxxx://xxxxxxxxxx.xxxxxxx.xx/). Abstractul lucrarii a aparut in volumul de rezumate al conferintei.