Colloidal NM. A selection of the (certified) reference materials and representative near-spherical test materials, with varying amplitude contrast and ranging from 10 to 200 nm in size: ERM-FD304 (nanosilica), ERM-FD100 (nanosilica), RM8012 (colloidal gold NIST), RM8013 (colloidal gold NIST), RM8011 (colloidal gold NIST), NM-300K (nanosilver) and tailor-made spherical and monodisperse SiO2NPs@IIT and AgNPs@IIT. • Non-spherical colloidal NM with a rod-shaped morphology (Au nanorods) • Tailor made mixtures composed of NM populations of spherical amorphous silica nanoparticles of different sizes and concentration (provided by IIT from HiQNano, xxxx://xxx.xxx-xxxx.xxx). They will be tested using the initial proposed SOPs. Following a hypothesis driven research approach, a mixture of different sizes (e.g., nominal 25, 50 and 115 nm) will be produced to test the reliability of the previous mentioned SOPs.