Confocal light sheet microscopy Sample Clauses

Confocal light sheet microscopy. In contrast to X-ray-based imaging, multi-channel confocal light sheet microscopy allows for simultaneous acquisition of vascular features and further fluorescent labelled structures of the same sample in a single scan with perfect alignment (no need for further registration). Our current acquisition protocol uses an isotropic resolution of 0.52x0.52x2µm3. The most limiting factor is PSF anisotropy resulting in critical axial resolution. Further improvement of the axial PSF, optimisation of the beam profile, and implementation of a virtual slit aperture is required to achieve sufficient spatial resolution for vascular reconstruction. Even though many classical optical imaging methods provide high spatial accuracy, they are restricted to the investigation of (brain) tissue within a depth of a few hundreds of microns because of the limited penetration ability of the illuminating radiation. Therefore, imaging larger samples requires sectioning to obtain specimens of manageable thickness, as in all-optical histology. Sectioning, however, can severely distort or even damage the biological tissue, which requires non-trivial reconstruction.