Context of the peer Sample Clauses

Context of the peer reviewed, NANoREG approved publications resulting from D2.10 The results of D2.10 were published in several peer-reviewed, NANoREG approved publications. Their context is given below. In the publication of Xxxx et al. [18] detailed background information regarding the Physical characterization of nanomaterials in dispersion by transmission electron microscopy in a regulatory framework is reviewed. It is shown that TEM is one of the few techniques that can identify nanoparticles according to the current definitions of nanomaterials. The different steps required to analyse dispersed nanomaterials by TEM are described in detail and related to existing literature. Methodologies to obtain homogeneous and stable dispersions of colloidal nanomaterials and powders are presented. The preparation of TEM specimens to obtain a representative distribution of particles on the grid is discussed. The application of TEM imaging methods, electron diffraction and analytical TEM to obtain complementary information on the size, morphology, crystallographic structure, electronic structure and composition of nanomaterials is reviewed. In a qualitative TEM analysis the key properties of the physical form of the nanomaterial under which it is exposed to in vitro and in vivo test systems are described based on TEM micrographs. Subsequently, a quantitative analysis which includes detection, classification and measurement of primary particle properties and validation of the measurement results can be performed. The possibility to extract 3D information by fractal analysis of electron micrographs of aggregated nanomaterials with a fractal-like structure is explored. In the publication of Xx Xxxxxxxxx et al. [23] detailed background information is given regarding the calculation of measurement uncertainties of size, shape, and surface measurements using transmission electron microscopy of near-monodisperse, near-spherical nanoparticles. The different steps required to determine the measurement uncertainties of dispersed nanomaterials by TEM are described in detail and related to existing literature. In this publication Transmission electron microscopy (TEM) is combined with a systematic selection procedure for unbiased random image collection, semi-automatic image analysis and data processing and validated for size, shape and surface topology measurements of silica nanoparticles. This method relies on a high level of automation of calibration, image acquisition, image analysis and data...
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