Microsatellites Clause Samples

Microsatellites. The total number of loci amplified and genotyped in 625 raccoons was 4,333 (8,666 alleles) with an overall missing data percentage of 0.96%. Complete microsatellite profiles were generated for 583 raccoons. The remaining 42 raccoons were missing data at one locus. Micro-Checker indicated the possibility of null alleles at 3 loci (PFL9 = 0.18, PFL11 = 0.16, G10X = 0.07), and allele frequencies were adjusted to account for possible genotyping errors. Microsatellites were amplified and scored consistently as no differences were observed in genotypes for the subset of 63 individuals examined twice (882 loci; 1,764 alleles). Table 3.1 Allelic diversity and size range for all 7 loci. Locus # Alleles Size Range P135 ▇▇ ▇▇▇-▇▇▇ P140 ▇▇ ▇▇▇-▇▇▇ P161 ▇▇ ▇▇▇-▇▇▇ G10X ▇▇ ▇▇▇-▇▇▇ PFL4 ▇▇ ▇▇▇-▇▇▇ PFL9 ▇▇ ▇▇▇-▇▇▇ PFL11 ▇▇ ▇▇▇-▇▇▇ Average 16.857 n/a The number of alleles at each locus ranged from 10 to 29, with an average of 16.857 alleles per locus (Table 3.1). No statistically significant (p > 0.05) instances of linkage disequilibrium were identified (data not shown).
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Microsatellites. In total, 1,272 loci (2,544 alleles) were genotyped in 182 raccoons. Two individuals failed to amplify at a single locus: one for locus P135 and one for locus PFL4. Micro-Checker indicated the possibility of null alleles at 3 loci (P135 = 0.06, PFL11 = 0.13, G10X = 0.09), and allele frequencies were adjusted to account for possible genotyping errors. Of the 25 replicate samples, 700 alleles were amplified and scored consistently; genotypes were consistent in replicates with no differences in allele sizes among any of the duplicate samples. Overall, the number of alleles at each locus ranged from 3-25 with an average of 12.9 alleles per locus (Table 2.2). Table 2.2 Microsatellite allelic diversity for all 7 loci. Locus # Alleles Size Range P135 ▇▇ ▇▇▇-▇▇▇ P140 ▇▇ ▇▇▇-▇▇▇ P161 ▇▇ ▇▇▇-▇▇▇ G10X ▇ ▇▇▇-▇▇▇ PFL4 ▇▇ ▇▇▇-▇▇▇ PFL9 ▇▇ ▇▇▇-▇▇▇ PFL11 ▇▇ ▇▇▇-▇▇▇ Average 12.9 n/a ▇▇▇▇▇-▇▇▇▇▇▇▇▇ equilibrium could not be rejected (p > 0.05) based on the adjusted allele frequencies obtained from Micro-Checker. No statistically significant linkage disequilibrium (p > 0.05) was detected among any of the markers (data not shown).
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