Preparation of samples for Western blotting Sample Clauses

Preparation of samples for Western blotting. All samples were diluted to 3 µg of protein/µL using RIPA buffer before one volume of Laemmli sample buffer (Sigma-Xxxxxxx, Missouri, USA) was added (final protein concentration 1.5 µg/µL), mixed and heated to 100oC for 5 minutes using an Eppendorf heater (Grant Instruments, Shepreth, UK). This process denatures proteins and dissociates quaternary subunits by disrupting disulphide bridges and intramolecular bonds such as electrostatic interactions. As a consequence, protein separation during electrophoresis occurs primarily as function of protein size and not by quaternary structure.
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  • Radiation Therapy/Chemotherapy Services This plan covers chemotherapy and radiation services. This plan covers respiratory therapy services. When respiratory services are provided in your home, as part of a home care program, durable medical equipment, supplies, and oxygen are covered as a durable medical equipment service.

  • Selection of Subcontractors, Procurement of Materials and Leasing of Equipment The contractor shall not discriminate on the grounds of race, color, religion, sex, national origin, age or disability in the selection and retention of subcontractors, including procurement of materials and leases of equipment. The contractor shall take all necessary and reasonable steps to ensure nondiscrimination in the administration of this contract. a. The contractor shall notify all potential subcontractors and suppliers and lessors of their EEO obligations under this contract. b. The contractor will use good faith efforts to ensure subcontractor compliance with their EEO obligations.

  • Program Monitoring The Contractor will make all records and documents required under this Agreement as outlined here, in OEC Policies and NHECC Policies available to the SRO or its designee, the SR Fiscal Officer or their designee and the OEC. Scheduled monitoring visits will take place twice a year. The SRO and OEC reserve the right to make unannounced visits.

  • Anesthesia Services This plan covers general and local anesthesia services received from an anesthesiologist when the surgical procedure is a covered healthcare service. This plan covers office visits or office consultations with an anesthesiologist when provided prior to a scheduled covered surgical procedure.

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