Demineralization Примерни клаузи

Demineralization. Demineralization was carried out in dilute HCl solution. The mineral content in the exo- skeleton of crustacean is not the same for each species treatments. All species were treated with 7% HCl solution at ambient temperature with a solution to solid ratio of 10 ml/g. The resulting solid fraction was washed with distilled water until neutral pH was achieved. Then the demin- eralized samples were dried and weighed. The treatments with hydrochloric acid and their dura- tions (24-72 h) depend on the nature of species. The emission of CO2 gas was found to depend upon the mineral content of different species and the penetration in shell's mass. The CO2 emission was higher in the case of rapana, mussel and snail rather than with other species because the mineral content in the former was larger. to neutrality and with hot ethanol (10 ml/g). The purified chitin was dried at 50°C to constant weight. The protein content was determined from the weight differences of dematerialized sample and that of the chitin obtained after alkaline treatments.