Application of the SOPs to resolve different subpopulations of MNM in a multimodal mixtureT Sample Clauses

Application of the SOPs to resolve different subpopulations of MNM in a multimodal mixtureT. It is challenging to measure the size and shape properties of MNM that are polydisperse in size, particularly when the fraction of the smallest particles is small. As a proof-of-principle, it was demonstrated that the developed TEM-based SOPs allowed measuring the size and shape properties of the primary particles of a trimodal MNM where the fractions of the smaller particles are smaller than those of the larger particles. For this purpose, tailor-made MNM were produced by IIT (Italy). Table 5 and Figure 7 illustrate, for example, that similar ECD are measured for silica nanoparticles in a monomodal preparation as in a mixture combining these three preparations. Similar results were obtained for other size and shape parameters (not shown). Name Mode Median Mean N Ag@IIT NPs 20.3 nm 20.4 nm 20.0 nm 39589 SiO2@IIT NPs 25 nm 25.2 nm 25.2 nm 27.2 nm 17953 SiO2@IIT NPs 50 nm 51.4 nm 51.6 nm 53.6 nm 5774 SiO2@IIT NPs 115 nm 119.4 nm 118.6 nm 118.4 nm 2712 SiO2@IIT Mix NPs 00-00-000 nm • 25 nm < 30 nm 23.6 nm 22.9 nm 22.6 nm 10 • 50 nm > 30 nm < 65 nm 51.4 nm 49.9 nm 48.8 nm 112 • 115 nm > 65 nm 112.3 nm 112.4 nm 111.6 nm 424 80 Number of particles 60 40 20 0 0 50 100 150 200