BCA Assay Sample Clauses

BCA Assay. Total protein concentrations in samples were determined using a BCA Protein Assay kit (Thermo Scientific) following the manufacturer’s instructions: 10 µl of each standard and unknown samples were pipetted into microplate xxxxx in duplicate. 200 µl of working reagent were added to each well with gentle mixing of the plate. The plate was then covered and incubated at 37 οC in a humidified incubator with 5 % CO2 in air for 30 minutes and then cooled to room temperature. The absorbance was measured at or near 562 nm on a plate reader.
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Related to BCA Assay

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