Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay.
2. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
3. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel.
4. Place a SPIFE Blotter C on the gel with the longer edge parallel with gel blocks. Gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and remove the blotter.
5. Clean the electrodes with deionized water before and after each use. Wipe with a lint free tissue.
6. Place a carbon electrode on the outside ledge of each gel block outside the magnetic posts. Improper contact between electrode and the gel block may cause skewed patterns. Close the chamber lid.
7. Press the TEST SELECT/CONTINUE button located on the Electrophoresis and Stainer sides of the instrument until the HIGH RESOLUTION option appears on the display.
Gel Preparation. 1. Carefully cut open the end of the gel pouch. Remove one gel from the protective packaging. Fold and tape the open end of the pouch to prevent drying of the gel. Remove the gel from the plastic mold and discard the mold.
2. Using a QuickGel Blotter C, gently blot the entire gel using slight fingertip pressure on the blotter. Remove the blotter.
Gel Preparation. 1. Carefully cut open the end of the gel pouch. Remove one gel from the protective packaging. Fold and tape the open end of the pouch to prevent drying of the gel. Remove the gel from the plastic mold and discard the mold.
2. Place a QuickGel Blotter C on the gel with the longer edge parallel with gel blocks. Gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and remove the blotter.
3. Dispense approximately 1 mL of REP Prep onto the left side of the rectangle on the electrophoresis chamber floor.
4. Place the edge of the gel next to the left edge of the marked rectangle. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right side. Make sure no bubbles remain under the gel.
5. Use lint-free tissue to wipe around the edges of the plastic gel backing to remove excess REP Prep.
6. Thoroughly wash electrodes with deionized water before and after each use. Wipe the QuickGel Electrode with a lint-free tissue. The Stainless Steel Electrode must be patted dry because of the rough surface. Ensure that the endcaps are screwed on tightly. The Stainless Steel Electrode must be replaced after use on 50 gels. Unscrew the endcaps from the old electrode and screw them tightly onto the new electrode.
7. Place a QuickGel Electrode on the outside ledge of the left gel block (cathode end) inside the magnetic posts.
8. Place a QuickGel Stainless Steel Electrode on the outside ledge of the right gel block (anode end) of the gel inside the magnetic posts. Improper contact between the electrodes and the gel block can result in skewed patterns. Close the chamber lid.
9. Use the arrows under SEPARATOR UNIT to select the appropriate test. To check parameters, select test and press SETUP.
Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay. Using a SPIFE Blotter C, gently blot the entire gel using slight fingertip pressure on the blotter. Remove the blotter.
2. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
3. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right side, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel.
4. Thoroughly wash the electrodes with deionized water before and after each use. Wipe the carbon electrodes with a lint-free tissue.
5. Place the carbon electrodes on the outside ledge of the gel blocks, outside the magnetic posts.
6. Press the TEST SELECT/CONTINUE button located on the Electrophoresis and Stainer sides of the instrument until ALKALINE PHOS option appears on the display.
Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay.
2. Place a REP Blotter C on the gel with the longer end parallel with the gel blocks. Gently blot the entire surface of the gel using light fingertip pressure on the blotter and remove the blotter.
3. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
4. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right side, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Ensure no bubbles remain under the gel.
5. Thoroughly wash the electrodes with deionized water before and after each use. Wipe the carbon electrode with a lint-free tissue. The Disposable Stainless Steel Electrode must be patted dry because of the rough surface. Ensure that the endcaps are screwed on tightly. The Disposable Stainless Steel Electrode must be replaced after use on 50 gels. Unscrew the endcaps from the old electrode and screw them tightly onto the new electrode.
Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay.
2. Place a REP Blotter C on the gel with the longer end parallel with the gel blocks. Gently blot the entire surface of the gel using light fingertip pressure on the blotter and remove the blotter.
3. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
4. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left and ending on the right side, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel.
5. Clean and wipe the electrodes with lint-free tissue. Do the same for the Reagent Spreaders (glass rods).
6. Place a carbon electrode on the outer ledge of each gel block on the outside of the magnetic posts. Improper contact between the electrode and the gel block may cause skewed patterns. Close the chamber lid.
7. Place a Reagent Spreader (glass rod) on each inner gel block, inside the magnetic posts.
8. Use the arrows under SEPARATOR UNIT to select the appropriate test. To check parameters, select test and press SETUP.
Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay.
2. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
3. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel.
4. Place a REP Blotter C on the gel with the longer edge parallel with the gel blocks. Gently blot the entire gel using slight fingertip pressure on the blotter and remove the blotter.
5. Clean the electrodes with deionized water before and after each use. Wipe with a lint free tissue.
6. Place a carbon electrode on the outside ledge of each gel block outside the magnetic posts. Improper contact between electrode and the gel block may cause skewed patterns. Close the chamber lid.
7. Use the arrows under SEPARATOR UNIT to select the appropriate test. To check parameters, select test and press SETUP.
Gel Preparation. 1. Carefully open one end of the pouch and remove one gel from the protective packaging. Reseal the pouch with tape to prevent drying of the gel. Remove the gel from the plastic mold and discard the mold.
2. Place a QuickGel Blotter C on the gel with the longer edge parallel with gel blocks. Gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and remove the blotter.
3. Dispense approximately 1 mL of REP Prep onto the left side of the electrophoresis chamber.
4. Place the gel over the REP Prep inside the rectangle on the chamber floor. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right side.
5. Use lint-free tissue to wipe around the edges of the gel backing to remove excess REP Prep. Make sure the gel remains in place and that no bubbles remain under the gel.
6. Clean the QuickGel Electrodes and Reagent Spreaders with deionized water before and after each use. Wipe with a lint-free tissue.
7. Place a QuickGel Electrode on the outside ledge of each gel block inside the magnetic posts. Improper contact between the electrodes and the gel block can result in skewed patterns. Close the chamber lid.
8. Use the arrows under SEPARATOR UNIT to select the appropriate test. To check parameters, select test and press SETUP.
Gel Preparation. 1. Carefully open one end of the pouch and remove one gel from the protective packaging. Reseal the pouch with tape to prevent drying of the gel. Remove the gel from the plastic mold and discard the mold.
2. Using a QuickGel Blotter C, gently blot the entire gel using slight fingertip pressure on the blotter. Remove the blotter.
3. Dispense approximately 1 mL of REP Prep onto the left side of the electrophoresis chamber.
4. Place the gel over the REP Prep inside the rectangle on the chamber floor. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right side.
5. Use lint-free tissue to wipe around the edges of the gel backing to remove excess REP Prep. Make sure the gel remains in place and that no bubbles remain under the gel.
6. Clean the QuickGel Electrodes and Reagent Spreaders with deionized water before and after each use. Wipe with a lint-free tissue.
7. Place a QuickGel Electrode on the outside ledge of each gel block inside the magnetic posts. Close the chamber lid.
8. Press the TEST SELECT/CONTINUE button located on the Electrophoresis and Stainer sides of the instrument until the appropriate test name appears on the displays.
Gel Preparation. 1. Carefully open one end of the pouch and remove one gel from the protective packaging. Reseal the pouch with tape to prevent drying of the gel. Remove the gel from the plastic mold and discard the mold.
2. Using a QuickGel Blotter C, gently blot the entire gel using slight fingertip pressure on the blotter. Remove the blotter.
3. Dispense approximately 1 mL of REP Prep onto the left side of the electrophoresis chamber.
4. Place the gel over the REP Prep inside the rectangle of the chamber floor. Hold the gel so that the samples numbered 1 to 10 are turned to the left side of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right side. Use lint-free tissue to wipe around the edges of the gel backing to remove excess REP Prep. Make sure the gel remains in place and that no bubbles remain under the gel.
5. Clean the QuickGel Electrodes with deionized water before and after each use. Wipe with a lint-free tissue.
6. Place a QuickGel Electrode on the outside ledge of each gel block inside the magnetic posts. Improper contact between the electrodes and the gel blocks may cause skewed patterns. Close the chamber lid.
7. Press the TEST SELECT/CONTINUE button located on the Electrophoresis and Stainer sides of the instrument until the ACID HEMOGLOBIN or QG-ACID HEMOGLOBIN option appears on the display.
