Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay. 2. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber. 3. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel. 4. Place a SPIFE Blotter C on the gel with the longer edge parallel with gel blocks. Gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and remove the blotter. 5. Clean the electrodes with deionized water before and after each use. Wipe with a lint free tissue. 6. Place a carbon electrode on the outside ledge of each gel block outside the magnetic posts. Improper contact between electrode and the gel block may cause skewed patterns. Close the chamber lid. 7. Press the TEST SELECT/CONTINUE button located on the Electrophoresis and Stainer sides of the instrument until the HIGH RESOLUTION option appears on the display.
Appears in 1 contract
Samples: End User Agreement
Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay.
2. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
3. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the rightright side, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel.
4. Place a SPIFE Blotter C on the gel with the longer edge parallel with gel blocks. Gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and remove the blotter.
5. Clean the electrodes with deionized water before and after each use. Wipe with a lint lint-free tissue.
6. Place a carbon electrode on the outside ledge of each gel block outside the magnetic posts. Improper contact between the electrode and the gel block may cause skewed patterns. Close the chamber lid.
7. Press the TEST SELECT/CONTINUE button buttons located on the Electrophoresis and Stainer sides of the instrument until the HIGH RESOLUTION SERUM PROTEIN or URINE PROTEIN option appears on the display.
Appears in 1 contract
Samples: End User Agreement
Gel Preparation. 1. Remove the gel from the protective packaging and discard the overlay.
2. Place a REP Blotter C on gel with the longer end parallel with the gel blocks. Gently blot the entire surface of the gel using light fingertip pressure on the blotter, and remove the blotter.
3. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis SPIFE chamber.
34. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamberpin. Gently lay the gel down on the REP Prep, starting from the left side and ending on the rightright side, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, posts to remove excess REP Prep. Make sure that the gel lays flat in the chamber and that no bubbles remain under the gel.
4. Place a SPIFE Blotter C on the gel with the longer edge parallel with gel blocks. Gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and remove the blotter.
5. Clean the electrodes with deionized water and wipe with lint-free tissue before and after each use. Wipe with a lint free tissue.
6. Place a carbon electrode on the outside ledge of each gel block outside the magnetic posts. Improper contact between the electrode and the gel block may cause skewed patterns. Close the chamber lid.
7. Press the TEST SELECT/CONTINUE button buttons located on the Electrophoresis and Stainer sides of the instrument until the HIGH RESOLUTION ACID HEMOGLOBIN option appears on the displaydisplays.
Appears in 1 contract
Samples: End User Agreement
Gel Preparation. 1. Remove the gel from the protective packaging and discard overlay.
2. Dispense approximately 2 mL of REP Prep onto the left side of the electrophoresis chamber.
3. Place the left edge of the gel over the REP Prep aligning the round hole on the left pin of the chamber. Gently lay the gel down on the REP Prep, starting from the left side and ending on the right, fitting the obround hole over the right pin. Use lint-free tissue to wipe around the edges of the plastic gel backing, especially next to electrode posts, to remove excess REP Prep. Make sure no bubbles remain under the gel.
4. Place a SPIFE Using the REP Blotter C on the gel with the longer edge parallel with gel blocks. Gently C, gently blot the entire surface of the gel using slight fingertip pressure on the blotter, and blotter then remove the blotter.
5. Clean the electrodes with deionized water before and after each use. Wipe with a lint free tissue.
6. Place a carbon electrode on the outside ledge of each gel block outside the magnetic posts. Improper contact between electrode and the gel block may cause skewed patterns. Close the chamber lid.
7. Press the TEST SELECT/CONTINUE button located on the Electrophoresis and Stainer sides of the instrument until the HIGH RESOLUTION option appears on the display.
Appears in 1 contract
Samples: End User Agreement