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Citric Acid Destain Sample Clauses

Citric Acid Destain. Ingredients: After dissolution, the destain contains 0.3% (w/v) citric acid. Preparation for Use: Pour 11 L of deionized water into the Destain vat. Add the entire package of Destain. Mix well until completely dissolved. Storage and Stability: Store the Destain at 15 to 30°C. It is stable until the expiration date on the package. Signs of Deterioration: Discard if solution becomes cloudy.
Citric Acid Destain. Ingredients: After dissolution, the destain contains 0.3% (w/v) citric acid. Preparation for Use: Pour 11 L of deionized water into the Destain vat. Add the entire package of Destain. Mix well until completely dissolved. Storage and Stability: Store the Destain at 15 to 30°C. It is stable until the expiration date on the package. To prevent the formation of toxic vapors, sodium azide should not be mixed with acidic solutions. When discarding reagents containing sodium azide, always flush sink with copious quantities of water. This will prevent the formation of metallic azides which, when highly concentrated in metal plumbing, are potentially explosive. In addition to purging pipes with water, plumbing should occasionally be decontaminated with 10% NaOH. A SPIFE Touch must be used to electrophorese and dry the gel. The gels may be scanned on a densitometer such as the QuickScan Touch/2000 (Cat. No. 1690/1660). Refer to the appropriate Operator's Manual for detailed operating instructions. Specimen: Serum is the specimen of choice.
Citric Acid Destain. Ingredients: After dissolution, the destain contains 0.3% (w/v) citric acid. Preparation for Use: Pour 11 L of deionized water into the Destain vat. Add the entire package of Destain. Mix well until completely dissolved. Storage and Stability: Store the Destain at 15 to 30°C. It is stable until the expiration date on the package. Signs of Deterioration: Discard if solution becomes cloudy. Sodium Azide Warning To prevent the formation of toxic vapors, sodium azide should not be mixed with acidic solutions. When discarding reagents containing sodium azide, always flush sink with copious quantities of water. This will prevent the formation of metallic azides which, when highly concentrated in metal plumbing, are potentially explosive. In addition to purging pipes with water, plumbing should occasionally be decontaminated with 10% NaOH. INSTRUMENTS A SPIFE 2000/3000 or the QuickGel Chamber must be used to electrophorese the gels. A high quality scanning densitometer such as the QuickScan 2000 (Cat. No. 1660) may be used to scan the gels.
Citric Acid Destain. Ingredients: After dissolution, the destain contains 0.3% (w/v) citric acid. Preparation for Use: Pour 11 L of deionized water into the Destain vat. Add the entire package of Destain. Mix well until completely dissolved. Storage and Stability: Store the Destain at 15 to 30°C. It is stable until the expiration date on the package. Signs of Deterioration: Discard if solution becomes cloudy. A SPIFE 2000/3000 Analyzer (Cat. No. 1130 or 1088) must be used to electrophorese and incubate the gel. The gel can be scanned on a densitometer using a 595 nm filter or on the Quick Scan 2000 (Cat. No. 1660). Refer to the appropriate Operator’s Manual for detailed operating instructions.

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