Mobility of Gβ-YFP Sample Clauses

Mobility of Gβ-YFP. (A) Cumulative probability distribution of the square dis- placements cdf (r2) of Gβ-YFP on the apical membrane of developed Gβ-YFP/wt, recorded with a time interval of 50 ms between subsequent images. Data were fitted to a two compo- nent model (eq.2.2) (orange solid line; residuals are displayed in the lower part of the figure), resulting in a fraction (α) of slow Gβ subunits, and a fraction (1-α) of fast Gβ subunits. The two fraction model describes the experimental results well in all the experimental con- ditions described. For comparison, a one-fraction model fit (eq.2.1) is shown (dark yellow dashed line). (B) Cumulative probability distributions of the square displacements on the api- cal membrane of developed Gβ-YFP/wt cells after 50 (black), 100 (gray), and 300 ms (light gray) time lag. As expected the data shifts with time lag towards higher squared displace- ments, r2. (C) The characteristic mean squared displacements (MSD1) were plotted versus time lag for the first ten time lags (50-500 ms) for the fast fraction of Gβ-YFP in wt cells. The data was fit with a free-diffusion model (eq.2.3), yielding a diffusion constant of D1 =
Sample 1
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Related to Mobility of Gβ-YFP

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