Periphyton Processing Sample Clauses

Periphyton Processing. In the laboratory, excess site water from each settled sample was decanted. Gravel was rinsed free of periphyton, using DI water as rinsate in a large beaker. The sample was stirred so that periphyton fragments suspended, while the gravel settled to the bottom of the beaker. Then, the suspension was transferred into the mixer (leaving the gravel behind in the beaker). Once the gravel was isolated from the bulk of the algal material, DI was added to the beaker and swirled to dislodge any remaining algal material from the gravel. While the algae was in suspension, the liquid and algae was poured off while holding back the gravel. The gravel was inspected for any remaining periphyton fragments, and then discarded. Once the gravel was removed, the algal suspension was settled and the overlying water decanted, taking care to retain all particles. The sample was gently stirred with a metal spoon, then “unhomogenized” samples (i.e., not blended) were transferred into 2 clean scintillation vials. The samples were then thoroughly homogenized in a food mixer. The homogenized slurry was again sub-sampled for enzyme assay (20 mL) and “homogenized” taxonomy samples (2 vials, each 20 mL). Another subsample of the remaining slurry (150 mL) was transferred into a pre-weighed plastic cup and dried to constant weight at 65ºC. The remaining sample was transferred to a graduated cylinder to record the remaining volume. Periphyton samples were preserved in 4% formalin buffered with sodium borate for taxonomic identification and biovolume determination.
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Related to Periphyton Processing

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