Preparation for Use. The solution is ready for use as packaged. Storage and Stability: The solution should be stored at room temperature (15 to 30°C), and is stable until the expiration date indicated on the label. Signs of Deterioration: Discard product if it appears cloudy or contains particulates.
Preparation for Use. The gels are ready for use as packaged. Storage and Stability: The gels should be stored at room temperature (15 to 30°C), in the protective packaging and are stable until the expiration date indicated on the package. DO NOT REFRIGERATE OR FREEZE THE GELS.
Preparation for Use. Dilute 2.5 mL of Acetate Buffer Concentrate to 25 mL with deionized water and use immediately. Storage and Stability: The solution should be stored at room temperature (15 to 30°C), and is stable until the expiration date indicated on the label. Signs of Deterioration: Discard product if it becomes cloudy or contains particulates.
Preparation for Use. Dissolve the dry stain in 1 liter of 10% acetic acid and mix thoroughly. Fill the SPIFE stain vat. Storage and Stability: The dry stain should be stored at 15 to 30°C and is stable until the expiration date indicated on the package. The stain solution is stable for six months when stored at 15 to 30°C in a closed container. Signs of Deterioration: The diluted stain should be a homogeneous mixture free of precipitate.
Preparation for Use. Reconstitute S.A.R.P. with 1.0 mL of deionized water. Swirl gently. Allow approximately 10 minutes for complete dissolution before use. Storage and Stability: Lyophilized S.A.R.P. is stable until the expiration date indicated on the vial when stored at 2 to 8°C. The ristocetin cofactor activity of the reconstituted product is stable for eight hours at 2 to 8°C or 30 days at -20°C.
Preparation for Use. Reconstitute each vial with 5.0 mL of the Tris-Buffered Saline. Allow to stand approximately 20 minutes, then mix well. Vortexing for 1-5 minutes improves uniform suspension of platelets. An alternative method to vortexing is aspiration of the 5 mLs of platelet suspension into a 5 mL syringe with a 21 or 22G needle and forcibly returning the suspension to the vial 4 or 5 times. Storage and Stability: Helena Lyophilized Platelets are stable until the expiration date printed on the vial label when stored at 2 to 8°C. The reconstituted product is stable for 30 days when stored at 2 to 8°C. Before use, allow the reconstituted platelets to come to room temperature and resuspend platelets by mixing thoroughly.
Preparation for Use. To prepare for use, remove the plate from the protective packaging and allow the agarose to equilibrate to room temperature. Storage and Stability: Rocket Plates must be stored at 2 to 8°C and maintained in moist condition within the bag. DO NOT FREEZE. The plates are stable until the expiration date indicated on the package. Signs of Deterioration: Discard the plate if dry in appearance or if the xxxxx are not round. A crystalline appearance indicates the agarose has been frozen.
Preparation for Use. Reconstitute S.A.R.P. with 1.0 mL of deionized water. Swirl gently. Allow approximately 10 minutes for complete dissolution before use. Storage and Stability: Lyophilized S.A.R.P. is stable until the expiration date indicated on the vial when stored at 2 to 8°C. The reconstituted product is stable for four hours, and should be kept at 2 to 8°C during testing. Signs of Deterioration: Unreconstituted S.A.R.P. should appear as a light yellow, dry plug.
Preparation for Use. Reconstitute Reference Plasma by adding 0.5 mL deionized water. Swirl gently to mix. Allow to stand for 10 minutes before use for complete dissolution. Storage and Stability: When stored at 2-8°C, the Reference Plasma is stable until the expiration date indicated on the package. Reconstituted solution is stable for 8 hours when stored at 2-8°C. Signs of Deterioration: Unreconstituted Reference Plasma should appear as a light yellow, dry plug.
Preparation for Use. Dissolve the dry stain in 1 liter of 10% acetic acid and mix thoroughly. Fill the SPIFE stain vat. Storage and Stability: The dry stain should be stored at 15 to 30°C and is stable until the expiration date indicated on the package. The stain solution is stable six months when stored at 15 to 30°C in a closed container. Signs of Deterioration: The diluted stain should be a homogeneous mixture free of precipitate. INSTRUMENT A SPIFE 2000 or 3000 Analyzer must be used to electrophorese, stain, destain, and then dry the gels. The gels may be scanned on a separate densitometer such as the QuickScan 2000 (Cat. No. 1660). Refer to the Operator’s Manual for detailed instructions. SPECIMEN COLLECTION AND HANDLING Specimen: Fresh serum, urine, or CSF is the specimen of choice. Use of plasma will cause a fibrinogen band to appear as a distinct narrow band between the beta and gamma fractions. Storage and Stability: If storage is necessary, serum samples may be stored covered at 15 to 30°C for 4 days, 2 to 8°C for 2 weeks, or -20°C for 6 months.6 Urine or CSF samples may be stored covered at 2 to 8°C for up to 72 hours or at -20°C for one month. Urine Sample Preparation: Urine samples may be run diluted, neat, or concentrated. Shake samples to homogenize. Centrifuge desired volume at 2000 x g for 5 minutes. Remove supernatant and concentrate as follows: Total Protein (mg/dL) Conc. Factor < 50 100X 50 - 100 50X 100 - 300 25X 300 - 600 10X > 600 5X CSF Sample Preparation: CSF samples may be used after proper concentration (10 - 50X).
