Common use of Regeneration of transgenic plants Clause in Contracts

Regeneration of transgenic plants. 1. Transfer independent resistant calli to the N6-R plant regeneration medium (see Appendix C for N6-R). Add 50 mg/l paramomycin to N6-R medium. Each 100 x 20 mm dish contains 30 ml N6-R medium, 10 calli on each dish. Dishes are sealed with surgical tape. Culture calli at 28 °C under cold fluorescent light for 2 weeks. 2. Transfer calli to fresh N6-R medium. Do not add paramomycin to N6-R medium for this subculture. Culture calli at 28 °C under cold fluorescent light until shoots and roots are formed. 3. Transfer plantlets to Magenta boxes containing 30 ml MS-F medium (see Appendix D for MS-F). Grow plantlets at 28 °C under cold fluorescent light for 10-14 days. 4. Acclimate in vitro regenerated plants. Plant containers will be opened 4 days before transplanting in order to xxxxxx plantlets. 5. Transfer plants to pots or to soil. Label T0 plants systematically with CW ID and event number, e.g. CW00001-01. Generate and maintain greenhouse and field maps for all T0 plants. Note: transformed plants must be resistant to paramomycin, and non-transgenic seedlings are albinos when treated with kanamycin (Fig. 8).