RNA purification Sample Clauses

RNA purification. Total RNA was isolated from cell cultures using Qiagen™ RNeasy mini (1x105<5x106) kit. Pelleted cells were resuspended in 350ul RLT lysis buffer and homogenised using the Qiashredder™ spin column (Qiagen) and DNA removed by use of the RNAse-free DNAse set (Qiagen) according to the manufacturer’s instructions. Eluted RNA was quantified using a Nanodrop™ spectrophotometer and protein contamination determined via the 230:280nm ratio. RNA was either reverse transcribed immediately or stored at -80ºC.
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Related to RNA purification

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