Summary of the test. In preparation for the test, fill test containers with appropriate vol- umes of nutrient medium and/or test solution. Start the test by introducing algae into the test and control con- tainers in the growth xxxxxxxx. Envi- ronmental conditions within the growth xxxxxxxx are established at predetermined limits.
Summary of the test. In preparation for the test, test xxxxxxxx are filled with appropriate volumes of dilution water. If a flow- through test is performed, the flow of dilution water through each chamber is adjusted to the rate desired. In a static test, the test substance is introduced into each test chamber. In a flow- through test, the rate in which the test substance is added is adjusted to estab- lish and maintain the desired con- centration of test substance in each test chamber. The test is started by randomly introducing gammarids, which have been acclimated to the test conditions, into the test xxxxxxxx. Gammarids in the test xxxxxxxx are observed periodically during the test; the dead gammarids are removed and the findings recorded. Dissolved oxygen concentration, pH, temperature, and the concentration of test substance in test xxxxxxxx are measured at speci- fied intervals. Data collected during the test are used to develop concentra- tion—response curves and LC50 values for the test substance.
Summary of the test. In preparation for the test, fill the series, exclusive of controls, should be at the chemical’s detection limit. The upper value, for water soluble com- pounds, should be the saturation con- centration. No replicates are required; and nominal concentrations of the chemical are acceptable unless defini- tive testing is not required.
