Common use of Transplant seedlings to the field Clause in Contracts

Transplant seedlings to the field. 1. Herbicide resistant seedlings at 4-5 leaf stage will be transplanted to field, ten transgenic lines for each construct, fifteen plants for each line, one row wild type plants every eleven rows as shown in Figure 1. 2. Save and score at least 30 seedlings for herbicide tolerance after herbicide sensitive plants have clearly died. Store numbers of herbicide sensitive and resistant seedlings in the Data Collection Table (ANNEX II). 3. Field layout — the distance between rows is 26.7 cm and the distance between plants within a row is 13.4 cm. Two guard rows will be grown for each plot. Walk lane will be 50 cm wide. 4. A plot map or a screenhouse map (ANNEX II) will be used to track each plant in each experiment. Figure 1. Sample plot of transgenic plants. 5. Measurements to be taken for all T1 plants a. Flowering time: Number of days from transplanting to heading of the earliest panicle. b. Plant height: From the ground to the top of the tallest panicle. c. Effective tiller number: Number of tillers bearing a fertile panicle. d. Seed yield: Weight of seeds while still on panicles, including the or envelope. 6. General observations To be noted in cases where the frequency is higher than expected based on control plants. Use coded comments whenever possible to describe plants (see ANNEX IV, Data Collection Table). A phenotyping update should be presented monthly to the Collaboration Committee. a. Growth vigor: Normal (no entry), improved (1), poor (2) b. Leaf color: Normal (no entry), darker green (1), yellow-green (2) c. Fertility: Normal (no entry), reduced (1). d. Lodging tolerance: Normal (no entry), increased lodging (1). e. Resistance to pests and diseases: Normal (no entry), increased resistance (1), hypersensitive (2). f. Stress tolerance (drought, cold, heat): Normal (no entry), increased tolerance (1), hypersensitive (2). g. Flag leaf color at maturity: Normal (no entry), more green than controls (1), more yellow than controls (2). h. Flag leaf angle from vertical: Normal (no entry), decreased leaf angle (1), increased leaf angle (2). 7. Optional measurements for selected events (upon request from Ceres) a. Fertility2: Number of filled seeds divided by number of spikelets on a panicle (average of 3 panicles). b. Panicle size: Number of seeds per panicle, averaged from three panicles. c. Seed yield2: Weight of total seeds less panicles and bag for lines showing 10 percent increase. d. Seed fill rate: Number of days from flowering to when 50% of seeds are yellow.