Zeta potential Sample Clauses

Zeta potential. The surface charge of nucleic acid/lipid complexes is an important property that affects its interaction with cells and non-cellular components such as serum proteins (Xxxxx, X.X. and X.X. Xxxxxx, 2008). Dynamic light scattering in the presence of an electrical field can estimate the zeta potential of the complexes, as particles in suspension will migrate toward the electrode of opposite charge with velocity proportional to zeta potential. The zeta potential is used to assess the charge stability of a disperse system as it can give an estimate of the surface charge of particles in suspension. It has been reported that complexes with positive charge are formed when the cationic lipid is in excess in comparison to the nucleic acid and at these ratios the complexes are more transfection efficient in vitro (Xxxxxxxxx, E. and X. X. Xxxxxxx, 1996, Xxxxxxx, X. et al., 2000a). However, non-specific interactions between negatively charged components in vivo and the positively charged complexes affects the stability of the complexes. Several strategies have been developed to reduce these non-specific interactions, but the most common is the use of hydrophilic uncharged polymers such as polyoxyethylene glycol (PEG) attached to the lipid in order to shield the positive charge and provide a steric barrier (Xxx, M. and X.X. Xxx, 2005).
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Zeta potential. The zeta-potential described the charge that particles have at specific conditions in a specific medium. In NANoREG D2.09, it was proposed to replace the ECHA characterization request on the dissociation constant

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