Cell Culture Media and Serum Sample Clauses

Cell Culture Media and Serum. Media used in day-to-day culture of cell lines and primary human cells are described below and specific ingredients are prepared as described. Components are expressed either as a percentage (v/v) or by concentration (e.g. μg/ml). Foetal Calf Serum (FCS): Fetal Calf Serum was heat inactivated (HI) by agitated incubation at 56⁰C for 1h in a water bath followed by storage in appropriately sized aliquots at -20⁰C. Human AB serum: Serum was purchased from PAA laboratories (Yeovil), heat inactivated by agitated incubation at 56⁰C for 1h in a water bath followed by storage in appropriately sized aliquots at -20⁰C. Human serum is abbreviated to HS henceforth.
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