Sample Preparation and Hybridization Sample Clauses

Sample Preparation and Hybridization. The cell lines were cultured at cell confluence according to the corresponding ATCC datasheets. Total RNA samples were extracted using the miRNeasy kit (Qiagen Spa, Hilden, Germany) and quantified by ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). Using an Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA), RNA integrity was assessed on the basis of the RIN (RNA integrity number) factor, and the presence of low molecular weight RNA (5S) was verified. Labeling of total RNA samples was performed using the FlashTagTM Biotin RNA labeling Kit (Genisphere Inc., Hatfield, PA, USA) starting from 1 µg of total RNA. Briefly, the tailing reaction is followed by ligation of the biotinylated signal molecule to the target RNA sample. The labeling reaction is based on Genisphere proprietary 3DNA dendrimer signal amplification technology. Prior to array hybridization, total RNA labeling was verified using the enzyme-linked oligosorbent assay (ELOSA). After that, biotin-labeled samples were hybridized onto the arrays at 48 ◦C, then washed and stained using the Affymetrix Fluidics Station 450. Arrays were scanned with the GeneChip⃝c Scanner 3000 7G to acquire fluorescent images of each array and analyzed by use of GeneChip Operating Software (GCOS, version 1.2).
Sample 1
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Related to Sample Preparation and Hybridization

  • Vaccination and Inoculation (a) The Employer agrees to take all reasonable precautions, including in-service seminars, to limit the spread of infectious diseases among employees.

  • Surface Preparation Clean the surface to be treated of all dust, dirt, clay, grass, sod and any other deleterious matter before application of the asphalt surface treatment.

  • Professional Preparation and Scholarship The certificated classroom teacher exhibits, in his/her performance, evidence of having a theoretical background and knowledge of the principles and methods of teaching, and a commitment to education as a profession.

  • Joint Preparation The preparation of this Agreement has been a joint effort of the parties and the resulting documents shall not, solely as a matter of judicial construction, be construed more severely against one of the parties than the other.

  • EVALUATION AND MONITORING The ORGANIZATION agrees to maintain books, records and other documents and evidence, and to use accounting procedures and practices that sufficiently and properly support the complete performance of and the full compliance with this Agreement. The ORGANIZATION will retain these supporting books, records, documents and other materials for at least three (3) calendar years following the year in which the Agreement expires. The COUNTY and/or the State Auditor and any of their representatives shall have full and complete access to these books, records and other documents and evidence retained by the ORGANIZATION respecting all matters covered in and under this Agreement, and shall have the right to examine such during normal business hours as often as the COUNTY and/or the State Auditor may deem necessary. Such representatives shall be permitted to audit, examine and make excerpts or transcripts from such records, and to make audits of all contracts, invoices, materials, and records of matters covered by this Agreement. These access and examination rights shall last for three calendar years following the year in which the Agreement expires. The COUNTY intends without guarantee for its agents to use reasonable security procedures and protections to assure that related records and documents provided by the ORGANIZATION are not erroneously disclosed to third parties. The COUNTY will, however, disclose or make this material available to those authorized by/in the above paragraph or permitted under the provisions of Chapter 42.56 RCW without notice to the ORGANIZATION. The ORGANIZATION shall cooperate with and freely participate in any other monitoring or evaluation activities pertinent to this Agreement that the COUNTY finds needing to be conducted.

  • Site Preparation Contractor shall not begin a project for which the site has not been prepared, unless Contractor does the preparation work at no cost, or until Region 4 ESC includes the cost of site preparation in a purchase order. Site preparation includes, but is not limited to: moving furniture, installing wiring for networks or power, and similar pre-installation requirements.

  • Planning and Preparation 1. Uses established local and the Core Curriculum Standards and Cumulative Progress Indicators as well as established local and state curriculum objectives in planning lessons.

  • Preparations No more than three (3) preparations per professor per term shall be the goal of the District and the Association. If more than three (3) preparations are necessary to meet particular needs of students or to meet the unique needs of a department, such additional preparations will only be assigned by the appropriate administrator following discussion with all affected members of the department. When such additional preparations are necessary, they shall be distributed equitably among department members.

  • Joint Network Implementation and Grooming Process Upon request of either Party, the Parties shall jointly develop an implementation and grooming process (the “Joint Grooming Process” or “Joint Process”) which may define and detail, inter alia:

  • Introduction and Background 1.1 The purpose of this Schedule 2 (Contract Services and Contract Supplies) is to set out the characteristics of the Contract Services and/or Contract Supplies (as the case may be) and Funding that the Provider will be required to make available to all Contracting Authorities in relation to Lot 1 and/or Lot 2 (as the case may be) and to provide a description of what the Contract Services and/or Contract Supplies (as the case may be) and Funding will entail.

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