Xxxxxx and peripheral blood preparation Sample Clauses

Xxxxxx and peripheral blood preparation. After sacrifice, single cell suspensions were made of spleen by maceration through a cell strainer. Blood was collected by cardiac puncture from euthanised mice into Eppendorf tubes containing citrate-dextrose (Sigma, Poole, UK) as anticoagulant. Red blood cells were lysed using 1X RBC Lysis Buffer (Biolegend, UK) according to manufacturer’s instructions.
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Related to Xxxxxx and peripheral blood preparation

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