Materials and Methods. 86 2.1 PARTICIPANTS 87 We used baseline measurements from a convenience sample of participants in previous (3) and 88 ongoing cohort studies investigating the effects of rehabilitation on balance responses (Table 1). PD 89 participants were mild-moderate with bilateral symptoms (Xxxxx and Xxxx stage 2-3 (13)). All 90 participants provided written informed consent and all study procedures were approved by Institutional 91 Review Boards at the Georgia Institute of Technology and Emory University.
Materials and Methods. Description of Simulations. We used a lattice-based stochastic patch occu- pancy model (26) to simulate spatially explicit dynamics of large numbers of species with dissimilar ecological traits. The model assumes that different lattice cells may represent different habitat type, and that habitat type across the lattice may be spatially correlated (26). In the present simulations, we assumed a high degree of spatial correlation in habitat type (parameter ω = 2 in ref. 26). Interspecific interactions are not modeled, but each species has distinct ecological traits defined by five parameters: colonization rate (c), extinction rate (e), average dispersal distance (1/α), mean phenotype (φ), and niche width (γ). Colonization rate and extinction rate control the probability of a species populating an unoccupied lattice cell and the probability of extinction in an occupied cell in unit time, respectively. These probabilities are affected by the habitat type of the cell in relation to the mean pheno- type and niche width of the species (26). Cells with habitat type close to the species mean phenotype support populations best, whereas niche width controls the sensitivity of the species to habitat type. In the present simu- lations, a species typically reaches the stochastic stationary state from a few dozen to a few hundred time steps (26). We run the simulations for at least 500 steps to ensure that most species had reached the quasi-stationary state. Increasing the simulation time did not qualitatively alter the results, al- though it resulted in a few additional extinctions as expected due to sto- xxxxxxxxxx. Further details of the simulations are given in SI Text.
Materials and Methods. Materials Sample preparation Hemocytometry Flow imaging microscopy (FIM)
Materials and Methods. Participants and Recruitment
Materials and Methods. The Ethics Committee of Xxxx University approved the study protocol. Informed consent was obtained from all participants or participant´s guardian, after the nature of the procedures had been fully explained. This is a prospective observational study of real-time live ultrasound examinations of adnexal masses. Consecutive patients referred for an ultrasound examination and found to have an adnexal mass judged to need surgical removal were scanned according to the research protocol by sonologist 1 (PS) as part of the clinical ultrasound examination. A second ultrasound examination was carried out before surgery by sonologist 2 (LV). Both examiners used the standardized IOTA examination and measurement technique and the IOTA terminology (11) to describe their ultrasound findings and noted their results in a dedicated paper form. Sonologist 2 was blinded to the results of sonologist 1. Information on the clinical variables included in LR1 and LR2 (personal history of ovarian cancer, current hormonal therapy, age of the patient), was obtained at the preoperative ultrasound examination by sonologist 2. All patients were operated on within 90 days after the preoperative ultrasound examination performed by sonologist 2. The excised tissues underwent histological examination and tumors were classified according to the criteria recommended by the International Federation of Gynecology and Obstetrics (16). Borderline tumors were classified as malignant. The patients were examined in the lithotomy position with an empty urinary bladder (11). Abdominal ultrasound examination was added when needed. The ultrasound variables assessed with regard to interobserver reproducibility are shown in Table 1. The size of the lesion and that of its largest solid component were measured (largest diameter and mean of three orthogonal diameters) using calipers on the frozen ultrasound image. A color score was assigned on the basis of subjective assessment of the color content of the tumor scan at power Doppler ultrasound examination. A color score of 1 indicates absence of color Doppler signals, a color score of 2 a minimal amount of color Doppler signals, a color score of 3 a moderate amount of color Doppler signals and a color score of 4 a large amount of color Doppler signals in the tumor (11). The ultrasound systems used were GE Voluson 730 Expert or GE Voluson E8 (GE Healthcare, Xxxx, Austria) with a 5–9-MHz transvaginal transducer. For power Doppler ultrasound examinations the f...
Materials and Methods. Study Design Procedures
Materials and Methods. Experimental overview
Materials and Methods. Generation of molecular maps Generation of logical models
Materials and Methods. Participants
Materials and Methods. Patients Imaging Technique Image and Data Analysis The images and data obtained were anonymized and transferred to an image-processing workstation (Advantage Windows 4.2, GE Health- care). CTP data were analyzed by 3 independent readers, 2 expert and the other inexperienced in CTP imaging. Before their CTP analyses for this study, the first expert reader (expert reader A) had 5 years’ experience in CTP imaging, whereas the second expert reader (expert reader B) had 13 years’ experience in head and neck CT imaging and 3 years’ experience in CTP imaging. Each performed a first CTP anal- ysis en masse after all the data were collected and a repeated CTP analysis on the same anonymous dataset during a period ranging from 4 to 8 weeks (median, 6.8 and 6.5 weeks for expert readers A and B respectively), to allow evaluation of intraobserver agreement.
