Xxxxxx and Xxxxxxx X. X. Xxxx* Abstract: The reproduction of emergent behaviors in nature using reaction networks is an important objective in synthetic biology and systems chemistry. Herein, the first experimental realization of an enzymatic reaction network capable of an adaptive response is reported. The design is based on the dual activity of trypsin, which activates chymotrypsin while at the same time generating a fluorescent output from a fluorogenic substrate. Once activated, chymotrypsin counteracts the trypsin output by competing for the fluorogenic substrate and producing a non-fluorescent output. It is demonstrated that this network produces a transient fluorescent output under out- of-equilibrium conditions while the input signal persists. Importantly, in agreement with mathematical simulations, we show that optimization of the pulse-like response is an inherent trade-off between maximum amplitude and lowest residual fluorescence.
Xxxxxx and Xxxxxxx X. Xxxxxxx, as its true and lawful attorneys-in-fact and agents, with full power of substitution and resubstitution, for the Depositor or in the Depositor's name, place and stead, in its capacity as Depositor of the Trust, to sign any and all amendments (including post-effective amendments) to the 1933 Act Registration Statement and the 1934 Act Registration Statement and to file the same, with all exhibits thereto, and any other documents in connection therewith, with the Commission, the Exchange and administrators of state securities or blue sky laws, granting unto said attorneys-in-fact and agents full power and authority to do and perform each and every act and thing requisite and necessary to be done in connection therewith, as fully to all intents and purposes as the Depositor might or could do in person, hereby ratifying and confirming all that said attorneys-in-fact and agents or any of them, or their respective substitute or substitutes, shall do or cause to be done by virtue hereof.
Xxxxxx and Xxxxxxx X. Male, who acknowledged that they did sign the foregoing instrument and that the same was their free act and deed. /s/ Xxxxxxx Xxxxxxx -------------------------------- (Notary Seal) Notary Public My Commission Expires: XXXXXXX XXXXXXX, Notary Public State of Ohio My Commission Expires Jan. 26, 2004 [PARK VIEW FEDERAL SAVINGS BANK LETTERHEAD] January 29, 2001 Xx. Xxxxxxx X.. Male Park View Federal Savings Bank Corporate Center 00000 Xxxxxx Xxxx Xxxxx, XX 00000 RE: Amendment to Severance Agreement Dear Xxxxxxx, The purpose of this letter is to memorialize in writing certain changes to your Severance Agreement dated the 26th day of October, 1999 (the "Agreement"), which changes, we, PVF Capital Corp. (the "Corporation") and Park View Federal Savings Bank (the "Bank"), have agreed to. In accordance with the terms of the Agreement, particularly Section 13 ("Amendments") thereof, please signify your consent to these changes by executing this letter and returning it to the Bank. The changes we have agreed to are as follows:
Xxxxxx and Xxxxxxx X. XXXXXX, TRUSTEES XXXXXX FAMILY LIVING TRUST UNDER AGREEMENT DATED 10/15/2002
Xxxxxx and Xxxxxxx X. Xxxxxxxx, the initial Class II directors shall consist of Xxxxxx XxxXxxxxx Read, Jr., Xxxxxx Xxxxxx and Xxxxxxx Xxxx, and initial Class III directors shall consist of Xxxx X. Xxxxxxxx, Xxxxxx X. Xxxxxxxx and Xxxxxxx X. Xxxxx.
Xxxxxx and Xxxxxxx X. Xxxxxx; and
Xxxxxx and Xxxxxxx X. Xxxxxxxx. Borrower agrees to be liable for all sums either: (A) advanced in accordance with the instructions of an authorized person or (B) credited to any of Borrower’s accounts with Lender. The unpaid principal balance owing on this Agreement at any time may be evidenced by endorsements on this Agreement or by Lender’s internal records, including daily computer print-outs. Lender will have no obligation to advance funds under this Agreement if: (A) Borrower or any guarantor is in default under the terms of this Agreement or any agreement that Borrower or any guarantor has with Lender, including any agreement made in connection with the signing of this Agreement; (B) Borrower or any guarantor ceases doing business or is insolvent; (C) any guarantor seeks, claims or otherwise attempts to limit, modify or revoke such guarantor’s guarantee of this Agreement or any other loan with Lender; (D) Borrower has applied funds provided pursuant to this Agreement for purposes other than those authorized by Lender; or (E) Lender in good faith believes itself insecure.
Xxxxxx and Xxxxxxx X. Xxxxxx and Amendment No. 1 to the Voting Agreement dated July 1, 1996; (g) STOCK PURCHASE AND PLEDGE AGREEMENT dated June 10, 1996 by and between Xxxxxxx X. Xxxxxx, Trustee U/T/A dtd 05/25/93 FBO Xxxxxxx X. Xxxxxx, and Xxxxxxx X. Xxxxxx, Trustee U/T/A dtd 05/25/93 FBO Xxxxxxx X. Xxxxxx and Xxxxxxx X. Xxxxxx; and (h) STOCK PLEDGE AND ASSIGNMENT FOR SECURITY dated June 17, 1991 by and among Xxxxxxx X. Xxxxxx, Xxxxxxx X. Xxxxxx, individually and as trustees of the Xxxxxxx X. Xxxxxx Family Trust and the Xxxxxxx X. Xxxxxx Family Trust, and CCI.
Xxxxxx and Xxxxxxx X. Xxxxx Telecopy No.: (000) 000-0000 With copies to: XxXxxxxxx, Will & Xxxxx 000 Xxxx Xxxxxx Xxxxxx Xxxxx 0000 Xxxxxxx, Xxxxxxxx 00000-0000 Attention: Xxxxxxx X. Xxxxxx, P.C. Telecopy No.: (000) 000-0000 and XxXxxxxx & English, LLP Four Gateway Center 000 Xxxxxxxx Xxxxxx Xxxxxx, Xxx Xxxxxx Attention: Xxxx X. Xxxxx, Esq. Telecopy No.: (000) 000-0000 Any party may change its address for receiving notice by written notice given to the others named above. Notices shall be deemed given as of the date of receipt.
Xxxxxx and Xxxxxxx X. Xxxxxx may transfer registration rights granted hereunder as to shares of Registrable Stock acquired in their capacity as Investors hereunder, subject to Sections 8.13(a) and (b) hereof.