Antibody specificity Sample Clauses

Antibody specificity. Antibodies for RPPA need to be highly specific, i.e. they need to show one major band at the expected molecular weight in Western Blot. Antibody testing is an important part of our activity. We test antibodies on a panel of cell lines, grown in different conditions in order to activate a maximum of cell signaling pathways. We use human cell lines and one mouse cell line (NIH-3T3). We can therefore tell whether an antibody recognizes the murine protein, at least in NIH-3T3 cells. This can be useful for projects concerning mouse tissue. Important: we can demonstrate that our antibody performs well on our cell lines. However, we cannot guarantee that our antibody works as well on your samples, whatever they are. Therefore, RPPA needs to be considered as a screening tool. Interesting observations need to be validated by another method (e.g. Western Blot, IHC, …) on a selection of informative samples. Also, if you do not observe a result that you expected for a given protein, based on preliminary data, this might be because the used antibody is not specific enough. This list of validated antibodies evolves continuously and is available upon request or on xxxx://xxxxx.xxxxx.xx/ (inside Xxxxx only).
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