DNA Oligonucleotides Sample Clauses

DNA Oligonucleotides. Table 2-6 Oligonucleotides used in this study Name Sequence Use Purpose JHH15 TCTTCAATAACATATGTCACTGAATG ACTTCCTAAGTTCC Forward primer (Rtt109) Construction of pJHH14 JHH16 ATAAATATCAAGTCGACTCAAGTTTT AGGCAAGGCTTTAGC Reverse primer (Rtt109) Construction of pJHH14 JHH34 CTGCCAGGCGGCCGCCTATTATTCTA AGATGTGGTCTTC Reverse primer (Pif1) Construction of pJHH16 JHH36 TGACGTCGCTAGCATGAGTAGTCGTG GTTTCAGGT Forward primer (Pif1) Construction of pJHH16 JHH40 aactttaagaaggagatatacATGGAACAAA AACTCATCTCAGAAGAGGATCTGGAA CAAAAACTCATCTCAGAAGAGGATCT Ggcgcgtaccaaacagaccgcg 2x Myc insertion for H3 Construction of pJHH17 JHH41 cgcggtctgtttggtacgcgcCAGATCCTCTT CTGAGATGAGTTTTTGTTCCAGATCC TCTTCTGAGATGAGTTTTTGTTCCAT gtatatctccttcttaaagtt 2x Myc insertion for H3 Construction of pJHH17 AM132 GAGGCTACTGCGCCAATT Forward primer Confirming integration of pRS306 at 5’ AM133 CTCAACCCTATCTCGGTCTATTCT Reverse primer Confirming integration of pRS303 and pRS306 at 5’ AM134 GTGCTGCAATGATACCGCGAG Forward primer Confirming integration of pRS303 and pRS306 at 3’ AM135 GTTACTTGGTTCTGGCGAGG Reverse primer Confirming integration of pRS306 at 3’ AM136 CACGACGCTTTGTCTTCATTC Forward primer Confirming integration of pRS303 at 5’ AM137 GTAGCCGCCGTTGTTGTTA Reverse primer Confirming integration of pRS303 at 3’
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