Fabrication of the Bio Sample Clauses

Fabrication of the Bio. EGOFET with biotin recognition element Sensors were fabricated using the device geometry developed by XXX in WP1 including interdigitated gold source and drain electrodes with different channel lengths and widths. The organic semiconductor was functionalized with biotin using two procedures developed by UPD and UNIBA. In the first one, sensors included an organic semiconductor bearing biotin groups. Several strategies were followed to increase the hydrophilicity of the polymer in order to decrease non- specific interactions. The second approach consisted of sensors with biotinylated phospholipid layers covalently attached to P3HT-COOH surface. Either a P3HT-COOH15 copolymer synthesized by UPD or a radio frequency (RF, 13.56 MHz) plasma enhanced chemical vapour deposition (PE-CVD) process was used to attach carboxylic acid functionalities on P3HT. The biotinylated phospholipids were covalently attached to the carboxyl groups through the formation of amidic bonds using the EDC/NHS chemistry. Both sensors show good electrical performance, with a field-effect mobility of 10-3 cm2/Vs and a current on-off ratio around 102, in good agreement with what found in the literature.
Sample 1
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