Luciferase assay Sample Clauses

Luciferase assay. Firefly luciferase catalyses the conversion of a molecule of D-luciferin to an electronically ‘excited’ molecule of oxyluciferin when in the presence of magnesium, ATP and oxygen. As dead cells cannot catalyse this reaction, the number of viable tumour cells is proportional to the amount of luminescence detected. By this means, the magnitude of T-cell induced cytotoxicity could be quantified. Tumour cell viability was monitored by luciferase assays and 48 and/or 72 hours. D-luciferin was added at 150 mg/mL immediately prior to luminescence reading. In each case, tumour cell viability was calculated as follows: (absorbance or luminescence of tumour cells cultured with T cells/absorbance or luminescence of untreated monolayer alone) x 100%.
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