Overcoming Surfaces Conditions Sample Clauses

Overcoming Surfaces Conditions. In order to swarm, bacteria must be able to overcome surface tension and dehydration on the agar surface. P. mirabilis produces an acidic extracellular polysaccharide matrix (ECM), or slime, prior to differentiation (279). The ECM constitutes 20% of the mass in a lag phase colony. The composition of the ECM has partially been characterized. Exopolysaccharides (EPS) comprise 30% of the ECM and are predominated by two types of sugars: one of low molecular weight (~1 kDa) which contains rhamnose and glucose, and one of high molecular weight (~300 kDa) which consists of rhamnose, galactosamine, glucose, and mannose. Lipids comprise 15% of the ECM with a novel phenoglycolipid (PGL) being present. Additionally, an osmoprotectant, glycinebetaine, was found a constituent of the ECM (280). The presence of PGL may aid in the vasoelasticity of swarm colonies while the presence of small sugars and glycinebetaine in the ECM suggests P. mirabilis faces drastic osmotic conditions and prepare accordingly to support swarming (273). Moreover, the ratio of the two EPS sugars changes drastically following differentiation. Slime is not only used for colony expansion, but its constituents may be acting as signals for differentiation (273, 280, 281). Overall, the P. mirabilis ECM is a mixture of complex molecules, the proportions of which are altered at each phase of swarming to promote migration and hydration of the population.
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