Plasma and Blood Amino Acid Analysis Sample Clauses

Plasma and Blood Amino Acid Analysis. ‌ Plasma amino acids were measured at baseline and after one month of BH4 therapy. A fasting blood sample was drawn from each patient in a heparinized tube and assessed using a Biochrom 30 Amino Acid Analyzer (Biochrom Ltd, Cambridge, UK). During diet liberalization, patients were instructed to spot a filter paper weekly with finger-stick blood drops after an overnight fast. Filter paper amino acid concentrations were analyzed using liquid chromatography/tandem mass spectrometry (Waters 2795 HPLC system/Micromass Quattro micro; Waters Corporation, Milford, Massachusetts, USA), as previously described [181].
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