SDS-PAGE Sample Clauses

SDS-PAGE. Once the conjugates were made, they had a dark pink to red color depending on the concentration. However, visually inspecting the solution cannot accurately determine if protein has bound to the AuNPs. One method of determining conjugation is to run an SDS-PAGE gel. Free AuNPs do not run through an SDS-PAGE gel and, instead, they precipitate out of solution due to their lack of stabilization (Figure 4.2 Lane 5 and 6, Figure 4.3 Lane 5). On the other hand, when protein is bound to the AuNP, the conjugates move through the SDS Page gel. When comparing the different size AuNPs in the gel, it is clear that the 5 nm AuNP conjugates moved further than the 15 nm and 30 nm conjugates. This makes sense because the 5 nm conjugates are lighter than the. Even though the 30 nm AuNP conjugates seem to have stayed in the well, however a different gel composition may have allowed the 30-nm conjugate to move. However, the color of the conjugates when compared to color of the black free AuNPs in the well is sufficient enough in determining that the protein is bound to the AuNP. When a gel with conjugates was destained, it is clear that he preparation is successful because no free protein could be found the a stained. Figure 4.4 shows a stained gel for 15 nm and 30 nm conjugates. Since 5 nm conjugates are performed in a similar manner, it is fair to assume that the 5 nm conjugates also lack free protein.