Workflow Validation. 1 The system should offer instrumentation tuning possibilities. Workflow descriptions should be accompanied with test data samples that can be run at any time for validation purposes (i.e. a “make test”). 2 The system could introduce the notion of workflow execution “goodness” to allow comparison of workflow parameters tweaking and to verify workflows coherence on the long run.
Workflow Validation. To validate YeastAnalysis platform, a small scale experiment is performed to compare two cell cultures. The first sample belonging to a strain of cells having a mutation in the BMH1 gene and expressing Nha1 protein attached to a green fluorescent protein (GFP). This cell strain is labelled ∆bmh1 Nha1-GFP. The second sample is from a wildtype cell strain ex- pressing Nha1 protein attached to GFP. This strain is labelled BY4741 Nha1-GFP. A set of 18 images are acquired with a Zeiss LSM5 Exciter confocal microscope. From the segmen- tation, 188 cells are detected using our novel segmentation method on the bright-field image channels; i.e. Xxxxx Transform followed by Circular Shortest Path (HCSP). More details about this method are explained in Chapter 3. Ensuing segmentation, the cells are measured for various features and all the measurements are written to a CSV file. The measurements are then analyzed automatically to generate a report including graph charts and statistics.
