Comparison of CK and BCA assays for cardiac Sample Clauses

Comparison of CK and BCA assays for cardiac viability Figure 2.4 displays standard concentration calibration curves obtained using the CK and BCA assays. In all cases, CK activity and protein concentration were directly proportional to the colourimetric changes measured at 405 nm or 652 nm respectively. Figure 2.5 displays the CK activity and protein concentrations of perfusates from ischaemic and control hearts. To display the results more clearly, only the readings from the 5 min time point are displayed from the CK assay. There was a clear increase in CK activity and protein concentration from ischaemic/reperfused hearts compared to control hearts. These results confirm that either assay can be used to measure cardiac viability, it was therefore decided to use the BCA assay to detect cardiac protein release as a measure of viability in all heart perfusion studies. A Abs 405 nm 0.09 0.08 0.07 0.06 0 20 40 60 CK (mU/ well) 1 min (r2 = 0.957, y=0.0005x +0.0511) 2 mins (r2 = 0.902, y=0.0004x + 0.0513) 3 mins (r2 = 0.993, y=0.0003x + 0.9022) 4 mins (r2 = 0.997, y=0.0003x + 0.0511) 5 mins (r2 = 0.994, y=0.0004x + 0.0506) B r2 = 0.999 y=1.234x - 0.2809
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