Determination of individual dose toxicity Sample Clauses

Determination of individual dose toxicity. In order to determine the overall effect of each, individual, compound dose on the viability (MTT reduction) and ATP content (ATP assay) of a single cell line, a 1-way repeated-measures ANOVA using blocked, mean data for each experimental repeat, was performed with concentration as the independent variable and absorbance minus background (control xxxxx with no MTT solution) or luminescence minus background (control xxxxx with media only) as the dependent variable for the MTT and ATP assays respectively. Raw absorbance and luminescence values were used due to the requirement for ANOVA data to show an equal distribution of variability in the groups. Normalisation of data by expressing them as percentage of untreated (control) cells would, provided the control data were included, violate this assumption due to the fact that the untreated control would exist as 100% ± 0% (Lew 2007). Therefore, given that the aim of the test was to compare the effect of a particular dose versus untreated, raw data were used.
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